Sc 8205

Manufactured by Santa Cruz Biotechnology

The Sc-8205 is a laboratory instrument used for the separation and purification of biological molecules. It is designed to perform high-performance liquid chromatography (HPLC) analysis. The Sc-8205 provides precise control over parameters such as flow rate, pressure, and temperature to ensure accurate and reproducible results.

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Lab products found in correlation

Ab85679, by Abcam (1 mentions) Ab24584, by Abcam (1 mentions) Dm750, by Leica (1 mentions) Permount sp15 100, by Thermo Fisher Scientific (1 mentions) Ultravision quanto detection system tl 060 qhd, by Thermo Fisher Scientific (1 mentions) Probeon plus slides, by Thermo Fisher Scientific (1 mentions) Icycler iq5 system, by Bio-Rad (1 mentions) Ab9610, by Merck Group (1 mentions) Rna stat 60, by Tel-Test (1 mentions) Bx51 microscope, by Olympus (1 mentions) Hoechst, by Merck Group (1 mentions) Flouromount, by Merck Group (1 mentions) G3893, by Merck Group (1 mentions) Sp5 confocal microscope, by Leica (1 mentions)

Close spelling variants of this product

Anti-PAR-2 (C-17; sc-8205,

3 protocols using sc 8205

Histological Evaluation of Epidermal Barrier Function

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To evaluate epidermal thickening, the ear and dorsal skin of each mouse was obtained on day 21 and fixed in 10% neutral buffered formalin. Tissues were embedded in paraffin and sliced into 5-μm-thick sections. Sections were then transferred to probe-on-plus slides (Thermo Scientific, Carlsbad, CA, USA), and deparaffinized skin sections were stained with H&E. Mast cells in the skin were stained with toluidine blue. Some sections were stained for immunohistochemical markers using anti-filaggrin (1:1000; ab24584; Abcam), anti-involucrin (1:1000; ab68; Abcam), anti-loricrin (1:1000, ab85679, Abcam), anti-occludin (1:1000; 33-1500; Invitrogen), anti-PAR-2 (1:100; sc-8205, Santa Cruz Biotechnology), and anti-TSLP (1:500; NB110-55234; Novus Biologicals) antibodies. The staining procedure was performed with an Ultravision Quanto Detection System (TL-060-QHD; Thermo Scientific). The slices were washed with PBS, dehydrated and mounted in Permount (SP15-100, Thermo Scientific). All stained sections were then examined by light microscopy (DM750, Leica, Wetzlar, Germany) to assess histological changes. Morphometric analysis (immunostained area of the epidermis to each analyzed protein in relation to total area of the epidermis) was performed using ImageJ 1.51 software (National Institutes of Health, Bethesda, MD, USA). All histological examinations were analyzed in 3 sections/animal slices.

Kim Y.J., Choi M.J., Bak D.H., Lee B.C., Ko E.J., Ahn G.R., Ahn S.W., Kim M.J., Na J, & Kim B.J. (2018). Topical administration of EGF suppresses immune response and protects skin barrier in DNCB-induced atopic dermatitis in NC/Nga mice. Scientific Reports, 8, 11895.

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Quantification of PAR2 Expression in Spinal Cord Myelination

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To begin to address the significance of PAR2 to myelination of the spinal cord, we quantified the expression of PAR2 RNA in the spinal cord of mice at P0, 7, 21 or 45 using real time PCR (Radulovic et al., 2015 (link)). RNA was isolated using RNA STAT-60 (Tel-Test, Friendswood, TX) and stored at -70 C until the time of analysis. Amplification of the housekeeping gene 18S in the same RNA samples was used to control for loading. Real-time PCR amplification in each case was accomplished using primers obtained from Integrated DNA Technologies (Coralville, IA), or Applied Biosystems (Grand Island, NY), as detailed in Table 1, on an iCycler iQ5 system (BioRad, Hercules, CA). In addition, we localized PAR2 (sc-8205, RRID: AB_2101309, Santa Cruz, Santa Cruz, CA) to Olig2 (Ab9610, RRID:AB_10141047, Millipore, Temecula, CA) or CC-1-postive cells (adenomatous polyposis coli, OP80, RRID:AB_213434, Millipore, Temecula, CA) within the spinal cord white matter at the P21 peak of myelination, using immunofluorescence techniques. Stained sections were cover slipped with Hardset containing DAPI (Vector, Burlingame, CA) and digitally imaged (Olympus BX51 microscope, Olympus, Center Valley, PA). Counts were made of either Olig2 or CC-1+ cells with a DAPI stained nucleus within the entire dorsal column of at least 3 mice at each time point without knowledge of genotype.

Yoon H., Radulovic M., Walters G., Paulsen A.R., Kristen D., Starski P., Wu J., Fairlie D.P, & Scarisbrick I.A. (2017). Protease Activated Receptor 2 Controls Myelin Development, Resiliency and Repair. Glia, 65(12), 2070-2086.

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Immunodetection of PAR2, TRPV4, and GFAP in Hippocampus

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The following primary antibodies were used for immunodetection: goat anti-PAR2 (sc-8205, Santa Cruz, 1:25), rabbit anti-TRPV4 (ACC-124, Alomone Labs, 1:50), rabbit anti-PAR2 (APR-032, Alomone Labs 1:500) and mouse anti-GFAP (G3893, Sigma-Aldrich, 1:2000). Hippocampal sections (50 μm) were blocked in 10% normal horse serum in 0.1 M PBS/0.1% Triton for 1 h at room temperature (RT). After 24–48 h incubation at 4°C with the primary antibody (together with 2% normal horse serum), sections were exposed to the appropriate secondary antibody (DyLight^TM 488 conjugated affinity purified donkey anti-goat IgG, 1:800; Alexa Fluor 594 AffiniPure donkey anti-rabbit IgG, 1:2000; Alexa Fluor 488 conjugated AffiniPure donkey anti-mouse IgG, 1:400) for 1 h. The sections were then washed, incubated with Hoechst (b1155, Sigma-Aldrich, 1 μg/ml final concentration) for 10 min (to allow nuclear staining), mounted on dry gelatin-coated slides and finally mounted and cover slipped with Flouromount (F4680, Sigma-Aldrich). Slides were imaged with a Leica SP5 confocal microscope and data were acquired and analyzed using a computer assisted image analysis system.

Shavit-Stein E., Artan-Furman A., Feingold E., Ben Shimon M., Itzekson-Hayosh Z., Chapman J., Vlachos A, & Maggio N. (2017). Protease Activated Receptor 2 (PAR2) Induces Long-Term Depression in the Hippocampus through Transient Receptor Potential Vanilloid 4 (TRPV4). Frontiers in Molecular Neuroscience, 10, 42.

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