Sodium dihydrogen phosphate

Tetrahydrocurcumin was kindly provided as a gift sample by Sanat Pharmaceuticals Ltd. (New Delhi, India). Carbopol^® 934, disodium hydrogen phosphate, sodium dihydrogen phosphate and Tween 80 were purchased from Central Drug House (P) Ltd. (New Delhi, India). Compritol^® 888ATO and Phospholipon 90 G were received as a gift sample from Panacea Biotec (Lalru, Punjab, India) and Gattefosse SAS, France, respectively. Chloroform and diethyl ether were purchased from Sisco Research Laboratories (Mumbai, India). Ethylene diamine tetra acetic acid and triethanolamine were provided by SD Fine Chemicals (Mumbai, India). Formalin and hydrochloric acid were supplied by Qualigens Fine Chemicals (Mumbai, India). Ellman reagent and 2,4-dinitro-chlorobenzene were obtained from Himedia Laboratories Ltd. (Mumbai, India). Methanol and polyethylene glycol were obtained from Fisher Scientific (Mumbai, India). 5,6-Carboxyfluoroscein was purchased from Sigma-Aldrich, Co., Burlington, MA, USA. The RayBio^® Mouse IL-6 ELISA Kit was provided by Ray Biotech, Inc., Norcross, GA, USA.

α-MNG (MW 410.46 g/mol; purity = 98%) was obtained from Sigma-Aldrich. PLGA [poly(D, L-lactide-co-glycolide)] lactide: glycolide (50:50) (Mw = 44,628 g/mol; viscosity, 0.71–1.0 dl/g) (Merck, India). Polyvinyl alcohol (PVA) was obtained from Sisco Laboratory (Mumbai, India) and Rhodamine B (RhB) from Sigma-Aldrich (St. Louis, MO, USA). The laboratory solvents, namely acetone, dichloromethane, acetonitrile, and deionized water, were procured from SD Fine Chemicals (Mumbai, India). LC-MS grade reagents, namely acetonitrile, methanol, ammonium acetate, ethyl acetate, glacial acetic acid, and water, were purchased from Sigma-Aldrich (St. Louis, MO, USA). The analytical grades of buffer reagents, i.e., phosphate-buffered saline (PBS), sodium dihydrogen phosphate, sodium hydroxide, disodium hydrogen phosphate, potassium dihydrogen phosphate, and ethanol, were procured from Central Drug House (New Delhi, India). For HPLC analysis, double distilled water was filtered through a micron-sized membrane filter (pore size, 0.45µ) and membrane filter (Durapore pore size, 0.21 µ) using an injectable needle.