Carboxyl PS (CPS) latex beads (20 and 200 nm; Invitrogen, Vienna, Austria), plain PS particles (PPS) (20 nm and 200 nm; ThermoScientific, Braunschweig, Germany), amine PS particles (AMI) 20 nm (Merck Chemicals and Life SCiences, Vienna, Austria) and 200 nm (Invitrogen), and amidine PS particles (APS) (20 nm and 200 nm, Invitrogen) were used. Aerosil®200 (Aero200; 12 nm) and Aerosil®OX50 (OX50; 40 nm) were obtained from Degussa, Frankfurt, Germany.
The human endothelial cell line EAhy926 (kind gift from Dr. C. J. Edgell) was cultured in Dulbecco minimal Eagle’s medium (DMEM), 10% fetal bovine serum (FBS), 2 mM L-glutamine and 1% penicillin/streptomycin. Human neuroblastoma cells SH-SY5Y (American Tissue and Cell Culture Collection, Manassas, VA, USA), used for the detection of intracellular [Ca2+] changes, were cultured in 90% DMEM/Ham’s F12, 10% FBS, 2 mM L-glutamine and 1% penicillin/streptomycin. All cells were cultured at 37 °C in a humid 95% air/5% CO2 atmosphere.
The human endothelial cell line EAhy926 (kind gift from Dr. C. J. Edgell) was cultured in Dulbecco minimal Eagle’s medium (DMEM), 10% fetal bovine serum (FBS), 2 mM L-glutamine and 1% penicillin/streptomycin. Human neuroblastoma cells SH-SY5Y (American Tissue and Cell Culture Collection, Manassas, VA, USA), used for the detection of intracellular [Ca2+] changes, were cultured in 90% DMEM/Ham’s F12, 10% FBS, 2 mM L-glutamine and 1% penicillin/streptomycin. All cells were cultured at 37 °C in a humid 95% air/5% CO2 atmosphere.