Isolated CD4+ T cells were incubated with anti-CD3 and anti-CD28 antibodies (10 µg/ml and 5 µg/ml respectively) for 30 min at 4ºC. Thereafter, anti-mouse Fc was added and incubated for an additional 30 min at 4°C and then immediately incubated at 37°C. After 15 min, cells were fixed, permeabilized and stained with mouse anti-human p38 (Becton–Dickinson®), following manufacturer’s instructions and analyzed in a FACScanto flow cytometer (BD Bioscience).
Mouse anti human p38
Manufactured by BD
The mouse anti-human p38 is a laboratory reagent used to detect and study the p38 mitogen-activated protein kinase (MAPK) in human samples. It is a primary antibody that specifically binds to the p38 protein, which plays a crucial role in various cellular processes, including stress response, inflammation, and cell differentiation.
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Lab products found in correlation
2 protocols using mouse anti human p38
1
Activation of p38 in CD4+ T cells
2
Activation and Analysis of p38 in CD4+ T Cells
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Isolated CD4 + T cells were incubated with anti-CD3/CD28 (10 µg/ml and 5 µg/ml respectively) during 30 min at 4ºC and then with anti-mouse Fc for additional 30 min at 4º and then immediately incubated at 37ºC. After 15min, cells were xed, permeabilized and stained with mouse anti-human p38 (Becton-Dickinson®) following manufacturer's instructions and analyzed in a FACScanto ow cytometer (BD Bioscience).
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