ICS assays were performed as previously described [46 (link)], with some modifications. Briefly, mouse splenocytes were added to the plate (2 × 106/well) and then stimulated with the peptide pool (2 μg/mL for individual peptide) for 5 h. PMA and ionomycin (Dakewe Bioengineering, China) were used as a positive control. The cells were incubated with GolgiStop (BD Biosciences, USA) for an additional 6 h at 37°C. Then, the cells were harvested and stained with anti-CD3 (BioLegend), anti-CD4 (BioLegend), and anti-CD8α (BioLegend) surface markers. The cells were subsequently fixed and permeabilized in permeabilizing buffer (BD Biosciences, USA) and stained with anti-mouse anti-IFNγ (BioLegend), anti-TNFα (BioLegend), anti-IL-2 (BioLegend), anti-IL-4 (BioLegend), and anti-IL-10 (BioLegend) antibodies. All fluorescent lymphocytes were gated on a FACSAria flow cytometer (BD Biosciences, USA).
Anti mouse anti ifnγ
Manufactured by BioLegend
Sourced in United States
Anti-mouse anti-IFNγ is a laboratory reagent used to detect and measure mouse interferon-gamma (IFNγ) in various applications, such as ELISA and flow cytometry. It specifically binds to mouse IFNγ, enabling its identification and quantification in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Permeabilizing buffer, by BD (2 mentions)
Anti cd8α, by BioLegend (2 mentions)
Anti tnf α, by BioLegend (2 mentions)
Anti il 2, by BioLegend (2 mentions)
Anti il 10, by BioLegend (2 mentions)
Anti cd3, by BioLegend (2 mentions)
Anti il 4, by BioLegend (2 mentions)
Anti cd4, by BioLegend (2 mentions)
Golgistop, by BD (2 mentions)
Facsaria ow cytometer, by BD (1 mentions)
Phorbol myristate acetate (pma), by Dakewe (1 mentions)
Ionomycin, by Dakewe (1 mentions)
Facsaria flow cytometer, by BD (1 mentions)
2 protocols using anti mouse anti ifnγ
1
Cytokine Profiling of Murine Splenocytes
2
Comprehensive Cytokine Profiling of Murine Splenocytes
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ICS assays were performed as previously described (90) , with some modifications.
Briefly, mouse splenocytes were added to the plate (2 x 10 6 /well) and then stimulated with the peptide pool (2 μg /ml for individual peptide) for 5 h. The cells were incubated with GolgiStop (BD Biosciences, USA) for an additional 6 h at 37°C. Then, the cells were harvested and stained with anti-CD3 (BioLegend), anti-CD4 (BioLegend) and anti-CD8α (BioLegend) surface markers. The cells were subsequently fixed and permeabilized in permeabilizing buffer (BD Biosciences, USA) and stained with anti-mouse anti-IFNγ (BioLegend), anti-TNFα (BioLegend), anti-IL-2 (BioLegend), anti-IL-4 (BioLegend) and anti-IL-10 (BioLegend) antibodies.
All fluorescent lymphocytes were gated on a FACSAria flow cytometer (BD Biosciences, USA).
Briefly, mouse splenocytes were added to the plate (2 x 10 6 /well) and then stimulated with the peptide pool (2 μg /ml for individual peptide) for 5 h. The cells were incubated with GolgiStop (BD Biosciences, USA) for an additional 6 h at 37°C. Then, the cells were harvested and stained with anti-CD3 (BioLegend), anti-CD4 (BioLegend) and anti-CD8α (BioLegend) surface markers. The cells were subsequently fixed and permeabilized in permeabilizing buffer (BD Biosciences, USA) and stained with anti-mouse anti-IFNγ (BioLegend), anti-TNFα (BioLegend), anti-IL-2 (BioLegend), anti-IL-4 (BioLegend) and anti-IL-10 (BioLegend) antibodies.
All fluorescent lymphocytes were gated on a FACSAria flow cytometer (BD Biosciences, USA).
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