Cyt420

Manufactured by Merck Group

Sourced in Ireland

The CYT420 is a laboratory instrument designed for cell analysis. It is capable of performing flow cytometry to detect and quantify various cell populations and their characteristics. The core function of the CYT420 is to provide researchers with a tool for analyzing complex biological samples and gaining insights into cellular biology.

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Lab products found in correlation

Ezhiasf 14k, by Merck Group (1 mentions)

2 protocols using cyt420

Confirmation Plasma Protein Profiling

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Proteins were selected for confirmation plasma studies from spots found to be altered by a fold-change ⩾1.1, based upon a 2-peptide minimum criterion and, in the first instance, having been previously implicated in depression^{22 (link), 23 (link), 24 (link)} or antidepressant treatment.^{11 (link), 25 (link)} Three proteins, for which immunoassay kits were readily available, were selected for confirmation and validation studies. PEDF and haptoglobin were selected from the proteins identified in the low-abundance gels, whereas serotransferrin was selected from the proteins identified in the high-abundance gels. The Phase Range Haptoglobin kit (TP801; TriDelta Development, Kildare, Ireland) was used to determine haptoglobin concentrations. Sandwich ELISA kits were used to measure serotransferrin (CSB-E13761h; Cusabio Biotech, Wuhan, China) and pigment epithelium-derived factor (PEDF) (CYT420; Millipore, Cork, Ireland) concentrations. The intra-assay % coefficient of variance for all assays was <10, whereas the inter-assay % coefficient of variance for PEDF was <20.

Ryan K.M., Glaviano A., O'Donovan S.M., Kolshus E., Dunne R., Kavanagh A., Jelovac A., Noone M., Tucker G.M., Dunn M.J, & McLoughlin D.M. (2017). Electroconvulsive therapy modulates plasma pigment epithelium-derived factor in depression: a proteomics study. Translational Psychiatry, 7(3), e1073-.

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Fasting Biomarkers Assessment Protocol

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A confirmed fasting blood sample was collected for measurement of selected outcomes in serum and plasma. Glucose was measured by the glucose oxidase method (YSI 2300 STAT plus, Yellow Springs, OH, USA). Insulin was measured using an enzyme-linked immunosorbent assay (ELISA) kit for human samples from Millipore (#EZHIASF-14K, St. Charles, MO, USA). The assay was 100% specific for human insulin according to the manufacturer’s specifications. Glucose and insulin values were used to calculate fasting insulin resistance using the HOMA-IR model (17 (link)). Serum triglycerides and total, HDL and LDL cholesterol, and C-reactive protein were measured at the Clinical Chemistry Laboratory of the Oklahoma Veterans Administration Hospital (Oklahoma City) using validated enzymatic assays (Synchron Systems, Beckman Coulter, Brea, CA, USA). Plasma PEDF was measured using an ELISA kit from Millipore (#CYT420).

Tryggestad J.B., Wang J.J., Zhang S.X., Thompson D.M, & Short K.R. (2014). Elevated plasma pigment epithelium-derived factor in children with type 2 diabetes mellitus is attributable to obesity. Pediatric diabetes, 16(8), 600-605.

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