The following compounds were used: dimethyl sulfoxide (DMSO) (Sigma-Aldrich) and bafilomycin A1 (Sigma-Aldrich). LysoTracker red DND-99 (Thermo Fisher Scientific) was used to label acidic organelles in live cells. Peptide-N-glycosidase F (PNGase F) (New England Biolabs) was used to remove N-linked oligosaccharides from proteins. For Western blotting and immunofluorescence, antibodies to the following proteins were used as primary antibodies: GAPDH (glyceraldehyde-3-phosphate dehydrogenase) (sc-25778; Santa Cruz Biotechnology), β-actin (sc-47778; Santa Cruz Biotechnology), HA tag (3724; Cell Signaling Technology), V5 tag (MCA1360; Bio-Rad), FLAG M2 tag (F1804; Sigma-Aldrich), 6×His tag (ab18184; Abcam), GFP (ab290; Abcam), epsin 2 (sc-376788; Santa Cruz Biotechnology), epsin 1 (sc-55556; Santa Cruz Biotechnology), CTSW (WH0001521M1; Sigma-Aldrich), calnexin (ab22595; Abcam), histone H3 (ab1791; Abcam), HA (35 (link)), NP (ATCC HB-65, H16-L10-4R5; American Type Culture Collection), and NA (PA532238; Thermo Fisher Scientific).
V5 tag mca1360
Manufactured by Bio-Rad
The V5 tag (MCA1360) is a protein tag commonly used in molecular biology and biochemistry applications. It is a small epitope tag derived from a glycoprotein found in the parainfluenza virus. The V5 tag can be added to recombinant proteins to facilitate their detection and purification using anti-V5 antibodies.
Automatically generated - may contain errors
Lab products found in correlation
Ab22595, by Abcam (2 mentions)
Epsin 2, by Santa Cruz Biotechnology (2 mentions)
Peptide n glycosidase f pngase f, by New England Biolabs (2 mentions)
Ab1791, by Abcam (2 mentions)
Ab18184, by Abcam (2 mentions)
Lysotracker red dnd 99, by Thermo Fisher Scientific (2 mentions)
Flag tag m2, by Merck Group (2 mentions)
Wh0001521m1, by Merck Group (2 mentions)
Ha tag, by Cell Signaling Technology (2 mentions)
Bafilomycin a1, by Merck Group (2 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (2 mentions)
Gapdh, by Santa Cruz Biotechnology (2 mentions)
β actin, by Santa Cruz Biotechnology (2 mentions)
2 protocols using v5 tag mca1360
1
Multimodal Protein Analysis with Diverse Reagents
2
Multimodal Protein Analysis with Diverse Reagents
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The following compounds were used: dimethyl sulfoxide (DMSO) (Sigma-Aldrich) and bafilomycin A1 (Sigma-Aldrich). LysoTracker red DND-99 (Thermo Fisher Scientific) was used to label acidic organelles in live cells. Peptide-N-glycosidase F (PNGase F) (New England Biolabs) was used to remove N-linked oligosaccharides from proteins. For Western blotting and immunofluorescence, antibodies to the following proteins were used as primary antibodies: GAPDH (glyceraldehyde-3-phosphate dehydrogenase) (sc-25778; Santa Cruz Biotechnology), β-actin (sc-47778; Santa Cruz Biotechnology), HA tag (3724; Cell Signaling Technology), V5 tag (MCA1360; Bio-Rad), FLAG M2 tag (F1804; Sigma-Aldrich), 6×His tag (ab18184; Abcam), GFP (ab290; Abcam), epsin 2 (sc-376788; Santa Cruz Biotechnology), epsin 1 (sc-55556; Santa Cruz Biotechnology), CTSW (WH0001521M1; Sigma-Aldrich), calnexin (ab22595; Abcam), histone H3 (ab1791; Abcam), HA (35 (link)), NP (ATCC HB-65, H16-L10-4R5; American Type Culture Collection), and NA (PA532238; Thermo Fisher Scientific).
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