The whole proteins were extracted using cell lysis buffer. Then, SDS-PAGE was used to separate proteins with different sizes. After that, protein was transferred to a PVDF membrane (Millipore) and target protein was immunoblotted with specific primary antibody. Primary antibodies were listed as follows: anti-E-cadherin (1:5000, mouse anti-human, Cell Signaling Technology Inc.), anti-Vimentin (1:2000, mouse anti-human, Abcam), anti-HPV16 E6 + HPV18 E6 (1:1000, mouse anti-human papillomavirus, Abcam), anti-HPV16 E7 (1:1000, mouse anti-human papillomavirus, Abcam), anti-YAP (1:2000, mouse anti-human, Proteintech), anti-TAZ (1:2000, mouse anti-human, Proteintech), anti-CCL2 (1:2000, mouse anti-human, Proteintech), anti-flag-YAP (1:1000, mouse, Abbkine), anti-GAPDH (1:2000, rabbit anti-human, Sigma-Aldrich). After incubating goat anti-rabbit or goat anti-mouse secondary antibody (MultiSciences), immunoblots were visualized using the chemiluminescence (ECL) reagent (Beyotime Biotechnology) and ChemiDoc XRS+ System (Bio-Rad).
Anti hpv16 e6 hpv18 e6
Manufactured by Abcam
Anti-HPV16 E6 + HPV18 E6 is a laboratory product that can be used to detect the presence of human papillomavirus (HPV) types 16 and 18 E6 proteins. These proteins are associated with the development of certain types of cancer. The product is designed for research use only and its core function is to provide a tool for researchers to study the role of HPV in disease processes.
Automatically generated - may contain errors
Lab products found in correlation
Pvdf membrane, by Merck Group (2 mentions)
Ecl reagent, by Beyotime (2 mentions)
Anti gapdh, by Merck Group (2 mentions)
Anti e cadherin, by Cell Signaling Technology (2 mentions)
Anti taz, by Proteintech (2 mentions)
Anti vimentin, by Abcam (2 mentions)
Chemidoc xrs system, by Bio-Rad (2 mentions)
Anti hpv16 e7, by Abcam (2 mentions)
Anti ccl2, by Proteintech (2 mentions)
Anti yap, by Proteintech (2 mentions)
2 protocols using anti hpv16 e6 hpv18 e6
1
Comprehensive Protein Analysis Protocol
2
Protein Extraction and Western Blot Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The whole proteins were extracted using cell lysis buffer. Then, SDS-PAGE was used to separate proteins with different sizes. After that, gel was transferred to a PVDF membrane (Millipore) and target protein was immunoblotted with speci c primary antibody. Primary antibodies were listed as follows: anti-Ecadherin (1:5000, mouse anti-human, Cell Signaling Technology Inc.), anti-Vimentin (1:2000, mouse antihuman, Abcam), anti-HPV16 E6 + HPV18 E6 (1:1000, mouse anti-human papillomavirus, Abcam), anti-HPV16 E7 (1:1000, mouse anti-human papillomavirus, Abcam), anti-YAP (1:2000, mouse anti-human, Proteintech), anti-TAZ (1:2000, mouse anti-human, Proteintech), anti-CCL2 (1:2000, mouse anti-human, Proteintech), anti-ag-YAP (1:1000, mouse, Abbkine), anti-GAPDH (1:2000, rabbit anti-human, Sigma-Aldrich). After incubating goat anti-rabbit or goat anti-mouse secondary antibody (MultiSciences), immunoblots were visualized using the chemiluminescence (ECL) reagent (Beyotime Biotechnology) and ChemiDoc XRS + System (Bio-Rad).
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