Il 10 and il 23 antibodies

All fluorogenic, non-fluorogenic dyes, Histopaque-1077 and standard platelet agonists were from Sigma Chemicals, USA. Monoclonal anti-cytochrome c antibody was from Epitomics, USA. z-DEVD-fmk, SB203580, ABT-737, Pifithrin-μ, antibodies against caspase-3, caspase-9, Bax, Bcl-2, BAD, phospho-BAD (ser136), Bid and tBid were from Santa Cruz Biotechnology, Inc., USA. Phospho-p38 (Thr180/Tyr182), p38, phospho-p53 (ser15), p53, COX-IV and β-tubulin antibodies were from Cell Signalling and Technology, USA. TNF-α, IL-1β and IL-6 antibodies were from Komabiotech, Korea. IL-10 and IL-23 antibodies were from Thermo Fisher Scientific, USA. LysoSensor green DND-189 was from Molecular probes, USA. Phenylhydrazine hydrochloride (PHZ) was from Fisher Scientific, USA. Commercial bilirubin and LDH estimation kits were purchased from Agappe Diagnostics Limited, India.

Freund’s complete adjuvant (FCA) containing 10 mg/mL heat-killed Mycobacterium tuberculosis, dihydrodichlorofluorescein diacetate (DCFDA), O-phthalaldehyde (OPT), HEPES and 1,1′,3,3′-tetramethoxypropane (TMP), dithiothreitol (DTT), dichlorofluorescein (DCF), hyaluronic acid sodium salt, gelatin (Type A from porcine skin), p-nitrophenyl β-N-acetyl glucosaminide, p-nitrophenyl β-glucuronide, reduced glutathione (GSH) and hemoglobin were procured from Sigma Chemicals, St. Louis, USA. TNF-α, IL-6 and IL-1β antibodies were from Cayman Chemicals, Michigan, USA, MMP-13, -3, -9, COX-2 and β-actin antibodies were from Epitomics, Inc. Burlingame, USA, IL-10 and IL-23 antibodies were from Thermo Fisher Scientific, New Hampshire, USA. GAPDH antibody was from were from Cell Signalling and Technology, Beverly, USA. Mini ELISA development kits for IL-6, IL-1β, TNF-α, and IL-10 were obtained from PeproTech, KHC Healthcare, India. Tamarind seeds were from local market, Mysore. All other chemicals used in this study were of analytical grade, purchased from Sisco Research Laboratories, Mumbai, India.