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Mca497bb

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The MCA497BB is a laboratory instrument designed for cell counting and analysis. It utilizes flow cytometry technology to detect and quantify specific cell populations within a sample. The device is capable of measuring various cellular parameters such as size, granularity, and the expression of specific surface markers. This information can be used to identify and characterize different cell types in a sample.

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Lab products found in correlation

Ab119843, by Abcam (2 mentions) Sudan red 3, by ITW Reagents (2 mentions) Ht110232 1l, by Merck Group (1 mentions) Sirius red, by Merck Group (1 mentions) Picric acid, by Merck Group (1 mentions)

2 protocols using mca497bb

1

Histopathological Analysis of Liver Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols
Liver samples were stained with Sudan Red III (251731.1606; Panreac, Castellar del Vallés, Barcelona, Spain) for lipid accumulation, Sirius Red (Direct Red 385548, Fast Green FCF:F7258, picric acid 197378; Sigma‐Aldrich) for fibrosis, and hematoxylin (HHS128‐4L; Sigma‐Aldrich) and eosin (HT110232‐1L; Sigma‐Aldrich), as described.18 Additionally, liver sections were immunostained for detecting F4/80 (MCA497BB; Bio‐Rad, Hercules, CA) and cluster of differentiation 64 (CD64; ab119843; Abcam, Cambridge, United Kingdom) for inflammation, as described.18 Quantification of staining areas was performed using FRIDA software and expressed as percentage of stained area.
Iruarrizaga‐Lejarreta M., Varela‐Rey M., Fernández‐Ramos D., Martínez‐Arranz I., Delgado T.C., Simon J., Gutiérrez‐de Juan V., delaCruz‐Villar L., Azkargorta M., Lavin J.L., Mayo R., Van Liempd S.M., Aurrekoetxea I., Buqué X., Delle Cave D., Peña A., Rodríguez‐Cuesta J., Aransay A.M., Elortza F., Falcón‐Pérez J.M., Aspichueta P., Hayardeny L., Noureddin M., Sanyal A.J., Alonso C., Anguita J., Martínez‐Chantar M.L., Lu S.C, & Mato J.M. (2017). Role of aramchol in steatohepatitis and fibrosis in mice. Hepatology Communications, 1(9), 911-927.
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2

Histological Analysis of Liver Tissue

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Liver samples were stained with Sudan red III (251731.1606; Panreac,
Castellar del Vallés, Barcelona, Spain) for lipid accumulation, Sirius
Red (Direct red -385548-, Fast green -FCF:F7258-, picric acid -197378-;
Sigma-Aldrich) for fibrosis and Hematoxylin (HHS128-4L; Sigma-Aldrich) and Eosin
(HT110232-1L; Sigma-Aldrich) (H&E) as described(18 (link)). Additionally, liver sections were
immunostained for detecting F4/80 (MCA497BB; Bio-Rad, Hercules, CA, USA) and
CD64 (ab119843; Abcam, Cambridge, UK) for inflammation as described(18 (link)). Quantification of staining
areas was performed using FRIDA software and expressed as stained area
percentage.
Iruarrizaga‐Lejarreta M., Varela‐Rey M., Fernández‐Ramos D., Martínez‐Arranz I., Delgado T.C., Simon J., Gutiérrez‐de Juan V., delaCruz‐Villar L., Azkargorta M., Lavin J.L., Mayo R., Van Liempd S.M., Aurrekoetxea I., Buqué X., Delle Cave D., Peña A., Rodríguez‐Cuesta J., Aransay A.M., Elortza F., Falcón‐Pérez J.M., Aspichueta P., Hayardeny L., Noureddin M., Sanyal A.J., Alonso C., Anguita J., Martínez‐Chantar M.L., Lu S.C, & Mato J.M. (2017). Role of aramchol in steatohepatitis and fibrosis in mice. Hepatology Communications, 1(9), 911-927.
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