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Anti rabbit igg h l hrp conjugated antibody

Manufactured by Promega
Sourced in United States

The Anti-rabbit IgG (H+L) HRP-conjugated antibody is a secondary antibody that binds to the heavy and light chains of rabbit primary antibodies. It is conjugated with horseradish peroxidase (HRP), which enables the detection of rabbit primary antibodies in various immunoassays.

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2 protocols using anti rabbit igg h l hrp conjugated antibody

1

Phosphorylation-Specific Antibody Assay

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TAK1 (phosphor-Ser192) (#orb7051) and NFKB1 (phosphor-Ser933) antibodies were purchased from Biorbyt (Cambridge, UK). The Anti-NF-kappaB p105 (pS932) phospho antibody (#MBS8210747) was purchased from MyBioSource (San Diego, CA). Mouse anti-chicken glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody (#AM4300), goat anti-mouse IgG horseradish peroxidase (HRP)-conjugated antibody (#A16078), and radioimmunoprecipitation assay (RIPA) lysis and extraction buffers were purchased from Thermo Fisher Scientific (Waltham, MA). Alexa Fluor 488 goat anti-rabbit IgG (H+L) secondary antibody and 4,6-diamidino-2-phenylindole (DAPI) were purchased from Invitrogen (#A-11008; Carlsbad, CA). Anti-rabbit IgG (H+L) HRP-conjugated antibody was purchased from Promega (#W4011; Madison, WI). The CD9 antibody was purchased from Cell Signaling (#13174; Danvers, MA).
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2

Immunodetection of Phosphorylated MAPKs

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Rabbit phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204, #4370) and rabbit phospho-p38 MAPK (Thr180/Tyr182, #4631) monoclonal antibodies were purchased from Cell Signaling (Danvers, MA, USA). Mouse anti-chicken GAPDH antibody was purchased from Thermo Fisher Scienti c (AM4300; Waltham, MA, USA). Alexa Fluor 488 goat anti-rabbit IgG (H + L) secondary antibody was purchased from Invitrogen (A-11008, Carlsbad, CA, USA). Anti-rabbit IgG (H+L) HRP-conjugated antibody was purchased from Promega (W4011; Madison, WI, USA). Goat anti-mouse IgG HRP-conjugated antibody (A16078) was purchased from Thermo Fisher Scienti c. In addition, HRP-conjugated rabbit anti-6-His antibody (A190-114P) was purchased from Bethyl Laboratories, Inc. (Montgomery, TX, USA) and 4′,6-diamidino-2-phenylindole (DAPI) was purchased from Invitrogen (Rockford, IL, USA). RIPA lysis and extraction buffers were purchased from Thermo Fisher Scienti c.
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