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Cytotox glotm cytotoxicity assay

Manufactured by Promega
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The CytoTox-Glo™ Cytotoxicity assay is a luminescent-based cell viability assay designed to quantify the number of dead cells present in a cell population. The assay measures a protease activity associated with cell membrane integrity, providing a sensitive and accurate method for determining cell cytotoxicity.

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Lab products found in correlation

Spark 10m microplate reader, by Tecan (1 mentions)

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CytoTox-Glo Cytotoxicity Assay (74 citations) CytoTox-Glo (35 citations) CytoTox-Glo assay (19 citations) CytoTox-Glo™ Cytotoxicity Assay kit (17 citations) CytoTox-GloTM Cytotoxicity Assay kit (5 citations) CytoTox-Glo Cytotoxicity (3 citations) CytoTox-Glo luminescent cytotoxicity assay (3 citations) Cytotoxicity assays (2 citations) CytoTox-Glo™ Cytotoxicity Assay Reagent (2 citations) Dead-Cell Protease Kit (CytoTox-Glo™ Cytotoxicity Assay from (2 citations)

2 protocols using cytotox glotm cytotoxicity assay

1

Cell Death Assessment in RBE4 Cells

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To evaluate cell death, RBE4 cells were seeded in 96-well plates coated with collagen and irradiated using broad-beam at different doses. The CytoTox-GloTM Cytotoxicity assay (Promega, Madison, USA) was used following the manufacturers protocol. The CytoTox-Glo™ Cytotoxicity Assay uses a luminogenic peptide substrate, the AAF-Glo™ substrate, to measure dead-cell protease activity, which is released from cells that have lost membrane integrity. Measurements were performed with a luminometer plate reader.
Hoorelbeke D., Decrock E., De Smet M., De Bock M., Descamps B., Van Haver V., Delvaeye T., Krysko D.V., Vanhove C., Bultynck G, & Leybaert L. (2020). Cx43 channels and signaling via IP3/Ca2+, ATP, and ROS/NO propagate radiation-induced DNA damage to non-irradiated brain microvascular endothelial cells. Cell Death & Disease, 11(3), 194.
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2

CytoTox-Glo Cytotoxicity Assay for Y-27632

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CytoTox-GloTM Cytotoxicity Assay (Promega, Fitchburg, WI, USA) was used to assess the cytotoxicity of Y-27632. Y-27632-treated SGSCs were cultured in 96-well plates for three days. Cytotoxicity assay solution (50 μL) was added to each well. The solution was mixed by orbital shaking, and plates were incubated at room temperature for 15 min. Luminescence was measured with a TECAN Spark 10M microplate reader (Tecan, Mannedork, Switzerland).
Kim K., Min S., Kim D., Kim H, & Roh S. (2021). A Rho Kinase (ROCK) Inhibitor, Y-27632, Inhibits the Dissociation-Induced Cell Death of Salivary Gland Stem Cells. Molecules, 26(9), 2658.
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