The largest database of trusted experimental protocols

Primerscript rt reagent kit perfect real time kit

Manufactured by Takara Bio
Sourced in China, Japan

The PrimerScript RT Reagent Kit Perfect Real Time Kit is a laboratory product designed for reverse transcription and real-time PCR. It includes reagents necessary for the conversion of RNA to cDNA and subsequent real-time quantitative PCR analysis.

Automatically generated - may contain errors

2 protocols using primerscript rt reagent kit perfect real time kit

1

Real-Time PCR Analysis of Overexpressed Genes

Check if the same lab product or an alternative is used in the 5 most similar protocols
The five overexpressed genes under chlorantraniliprole exposure were selected to determine their relative expression level by quantitative real-time PCR (qPCR) method at different developmental stages, within different tissues and dsRNA feeding of fall armyworm. Three replications were conducted for each sample. The designed primers for each specific genes were shown in Table 1. RNA was extracted as mentioned above, and 500-ng RNA with DNA-free was reverse-transcribed for synthesizing the first-strand cDNA using the PrimerScript RT Reagent Kit Perfect Real Time Kit (Takara, Dalian, China) based on the manufacturer’s instructions. The qPCR (20 µl) contained were performed in a mixture. The reaction was performed on an ABI 7500 Real Time PCR system (Applied Biosystems) with three biological replicates and two technical replications for each cDNA sample. GADPH was used as the reference gene for normalization of the expression levels (do Nascimento et al. 2015 (link)). The relative expression level for the selected target genes was calculated using the 2−ΔΔCT method (Livak and Schmittgen 2001 (link)).
+ Open protocol
+ Expand
2

Nrf2 and microRNA-380-3p Regulation in MPTP-Induced Neurodegeneration

Check if the same lab product or an alternative is used in the 5 most similar protocols
PQ dichloride hydrate (99.2% w/w) and MPTP (99.99% w/w) were purchased from Sigma-Aldrich Co. (St. Louis, MO). The terminal deoxynucleotidyl transferase-mediated dUTP biotin nick end-labeling (TUNEL) kit and MaxvisionTM (HRP-polymer anti-rabbit IHC kit) were purchased from Fuzhou Maixin Biotech. Co. Ltd. (China). Anti-Nrf2 (C-20, sc-722) and anti-TH (sc-374047) antibodies were purchased from Santa Cruz Biotechnology Inc. (Santa Cruz, CA). A Bulge-LoopTM miRNA QRT–PCR Primer kit was purchased from RiboBio Co. Ltd. (Guangzhou, China). A Primer Script RT Reagent Kit (Perfect Real-Time) kit and a SYBR Premix Ex Taq II kit were purchased from TaKaRa Biotechnology Co. Ltd. (Japan). A MicroRNA ISH Buffer, Controls Kit, and a LNA™ microRNA-380-3p probe were purchased from Exiqon Life Sciences, Life Sciences and Diagnostics Co. (Denmark). QRT–PCR primers for microRNA-380-3p were purchased from Guangzhou RiboBio Co., Ltd (China). Goat anti-Rabbit-DIG-AP IgG and NBT/BCIP staining solutions were purchased from Roche Co. (USA). Sheep serum was purchased from Millipore Corporation (Temecula, CA).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!