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Ceramide mid 15b4

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Ceramide MID 15B4 is a laboratory reagent used for research purposes. It is a synthetic ceramide compound that can be used as a standard or reference material in various analytical techniques.

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Lab products found in correlation

Sqstm1 p62, by Cell Signaling Technology (2 mentions) P mtors2448, by Cell Signaling Technology (1 mentions) Atg5 atg12, by Cell Signaling Technology (1 mentions) Beclin 1, by Cell Signaling Technology (1 mentions) Sphingosine 1 phosphate (s1p), by Merck Group (1 mentions) C6 ceramide, by Enzo Life Sciences (1 mentions) Gata2, by Santa Cruz Biotechnology (1 mentions) Sphk1, by Santa Cruz Biotechnology (1 mentions) P ulk1s758, by Cell Signaling Technology (1 mentions) C2 dihydroceramide, by Merck Group (1 mentions) C2 ceramide, by Santa Cruz Biotechnology (1 mentions) Atg13, by Cell Signaling Technology (1 mentions) Smase from staphylococcus aureus, by Merck Group (1 mentions) Gata1, by Santa Cruz Biotechnology (1 mentions) Pakt s473, by Cell Signaling Technology (1 mentions) Hpv16 e6, by Santa Cruz Biotechnology (1 mentions) Hpv16 e7, by Santa Cruz Biotechnology (1 mentions) Tom20, by Santa Cruz Biotechnology (1 mentions) Lamin b, by Santa Cruz Biotechnology (1 mentions)

Spelling variants of this product

MID 15B4 (2 citations) Anti-ceramide (MID 15B4) (2 citations) Anti‐ceramide antibody (clone MID 15B4, (2 citations)

2 protocols using ceramide mid 15b4

1

Erythroid differentiation regulation

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Desmethylimipramine (desipramine), N,N′-Bis[4-(4,5-dihydro-1H-imidazol-2-yl)phenyl]-3,3′-p-phenylene-bis-acrylamide dihydrochloride (GW4869), SMase from Staphylococcus aureus, C2 dihydroceramide, S1P, and BafA1 were purchased from Sigma-Aldrich (Bornem, Belgium), C2-ceramide from Santa Cruz Biotechnology (Boechout, Belgium) and C6-ceramide from Enzo Life Sciences (Antwerp, Belgium). Human recombinant TNFα was kindly provided by Pr. Athanase Visvikis (Université de Lorraine, Nancy, France). Stem cell factor (SCF) and interleukin (IL)-3 were purchased from ReliaTech (Wolfenbüttel, Germany). The erythroid differentiation is induced with human recombinant erythropoietin (Epoietin beta, Neorecormon Roche, Grenzach-Whylen, Germany). The primary antibodies used were directed against: GATA-1, GATA-2, PU.1, α-, β-, and γ-globin (Santa Cruz Biotechnology), SphK1, PI3K, P-PI3KY458, AKT, P-AKTS473, P-ULK1S758, mTOR, P-mTORS2448, Atg7, Atg5-Atg12, Atg13, P-Atg13S355, Beclin-1, p62/SQSTM1 (Cell Signaling, Leiden, The Netherlands), LC3 (Sigma), Rubicon (Abcam, Cambridge, UK), and ceramide MID 15B4 (Enzo Life Sciences, Antwerpen, Belgium), and the antibodies used for flow cytometry (CD235a/GPA, CD11b) were purchased from BD BioSciences (Erembodegem, Belgium).
Orsini M., Chateauvieux S., Rhim J., Gaigneaux A., Cheillan D., Christov C., Dicato M., Morceau F, & Diederich M. (2018). Sphingolipid-mediated inflammatory signaling leading to autophagy inhibition converts erythropoiesis to myelopoiesis in human hematopoietic stem/progenitor cells. Cell Death and Differentiation, 26(9), 1796-1812.
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2

Multiparametric Analysis of Autophagy Markers

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Tom20 (sc‐17764, Santa Cruz Biotechnology), CERS1 (sc‐65096, Santa Cruz Biotechnology); E2F5 (E‐19, sc‐999, Santa Cruz Biotechnology), HPV16 E6 (N‐17, sc‐1584, Santa Cruz Biotechnology), HPV16 E7 (NM2, sc‐65711, Santa Cruz Biotechnology); lamin B (C‐20: sc‐6216, Santa Cruz Biotechnology); clathrin (TD.1, sc‐12734, Santa Cruz Biotechnology); ceramide (MID15B4, Enzo Life Sciences); actin (A2066, Sigma, Inc.), Drp1 (BD# 611738, Clone 22/Drp1, BD Biosciences), ACO2 (#6922, Cell Signaling Technology); MFF (ab81127, Abcam); LC3 (D11 XP® mAb #3868, Cell Signaling Technology); ATG5 (#2630, Cell Signaling Technology); p53 (7F5, Rabbit mAb #2527, Cell Signaling Technology); pRb (4H1, Mouse mAb #9309, Cell Signaling Technology), SQSTM1/p62 (#5114, Cell Signaling Technology); SQSTM1/p62 (#5114, Cell Signaling Technology), SMCR7/MIEF2/MID49 (#PA5‐46624, Invitrogen).
Thomas R.J., Oleinik N., Panneer Selvam S., Vaena S.G., Dany M., Nganga R.N., Depalma R., Baron K.D., Kim J., Szulc Z.M, & Ogretmen B. (2017). HPV/E7 induces chemotherapy‐mediated tumor suppression by ceramide‐dependent mitophagy. EMBO Molecular Medicine, 9(8), 1030-1051.
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