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Mass spectrometric immunoassay msia tips 991cus01

Manufactured by Thermo Fisher Scientific

The Mass Spectrometric Immunoassay (MSIA) Tips (991CUS01) are a type of laboratory equipment used in analytical chemistry and biochemistry. The core function of these tips is to facilitate the capture and enrichment of target analytes from complex sample matrices for subsequent mass spectrometric analysis.

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2 protocols using mass spectrometric immunoassay msia tips 991cus01

1

Quantitative Apolipoprotein C Assay

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Polyclonal goat anti-human antibodies to apoC-I (Cat. No. 31A-G1b), apoC-II (Cat. No. 32A-G2b), and apoC-III (Cat. No. 33A-G2b) were obtained from Academy Biomedical (Houston, TX). Acetone (UN1090) was from JT Baker (Radnor, PA). Hydrochloric acid (HCl; AB06037), trifluoroacetic acid (TFA, AB02010), and acetonitrile (ACN; AB00120) were from AmericanBio (Natick, MA). N-methylpyrrolidinone (NMP; BP1172-4), 1,1ʹ carbonyldiimidazole (97%) (CDI, 115533), phosphate buffered saline buffer (PBS, 28372), 2-(N-morpholino) ethanesulfonic acid (MES) saline buffer (28390), and Mass Spectrometric Immunoassay (MSIA) Tips (991CUS01) were acquired from Thermo Fisher Scientific (Waltham, MA). Tween20 (Cat. No. P7949), sinapic acid (85429), and ethanolamine (ETA; 398136) were obtained from Sigma Aldrich (St. Louis, MO). ApoC-I (Cat. No. EA8011-1), C-II (EA8012-1), and C-III (EA8133-1) ELISA kits were acquired from Assay Pro (St. Charles, MO).
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2

Apolipoprotein E Antibody Characterization

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Polyclonal goat anti-human antibody to apoE (Cat. No. 50A-G1) was obtained from Academy Biomedical (Houston, TX). Acetone (UN1090) was from JT Baker (Radnor, PA). Hydrochloric acid (HCl; AB06037), trifluoroacetic acid (TFA; AB02010), and acetonitrile (ACN; AB00120) were from AmericanBio (Natick, MA). N-methylpyrrolidinone (NMP; BP1172-4), 1,1’ Carbonyldiimidazole (97%) (CDI; 115533), phosphate buffered saline buffer (PBS; 28372), 2-(N-Morpholino)ethanesulfonic acid (MES) saline buffer (28390), and Mass Spectrometric Immunoassay (MSIA)-Tips (991CUS01) were acquired from Thermo Fisher Scientific (Waltham, MA). Tween 20 (Cat. No. P7949), sinapic acid (85429), ethanolamine (ETA; 398136), and sialidase (Neuraminidase from Clostridium perfringens, N2876-6UN) were obtained from Sigma Aldrich (St. Louis, MO).
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