The 1% pentobarbital sodium was used to anesthetize mice, and kidney was removed. The renal tissues were fixed using 10% formalin and had paraffin embedding, the tissues were cut into 5 μm thick slices, and then, they were deparaffinized and rehydrated using xylene and ethanol. Next, the tissues were stained using HE stain (Solarbio, China) according to the manual of kit, and the images were captured by an optical microscope (Nikon, Japan).
He stain
Manufactured by Solarbio
Sourced in China
HE stain is a laboratory staining technique used to visualize cellular structures and tissues. It involves the use of two dyes, hematoxylin and eosin, which stain the nuclei and cytoplasm respectively, allowing for the differentiation of various cell types and tissue components.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using he stain
1
Histological Analysis of Murine Kidney
2
TBI Brain Tissue Staining and Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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At 72h after TBI, 3 complete brain tissues were taken from each group after anesthesia. The paraffin embedding and sectioning methods were similar to the previous immunofluorescence steps. The brain sections were stained with HE stain (Solarbio, Beijing, China), observed under a fluorescence microscope (Olympus, BX53). The brain defect area was calculated using the Image J (v8.0.1, SanDiego, USA) software. Tissue area loss was calculated by subtracting the ipsilateral area (damaged hemisphere) from the contralateral area (control hemisphere) 21 (link).
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