Monoclonal rabbit anti il 1β d3u3e

LPS, ATP, TPA, and dansylsarcosine piperidinium salt (DSS) were purchased from Sigma-Aldrich (St. Louis, MO, USA). RPMI-1640 and DMEM were obtained from Gibco (Grand Island, NY, USA). Nigericin and Ac-YVAD-cmk were obtained from InvivoGene Biotech Co., Ltd. (San Diego, CA, USA). The caspase-1 inhibitor VX-765 was purchased from Selleckchem (Houston, TX, USA). Antibody against Flag (F3165) (1:2000) and monoclonal mouse anti-GAPDH (G9295) (1:5000) were purchased from Sigma (St Louis, MO, USA). Monoclonal rabbit anti-NLRP3 (D2P5E) (1:1000), monoclonal rabbit anti-IL-1β (D3U3E) (1:1000), IL-1β mouse mAb (3A6) (1:1000), and monoclonal rabbit anti-caspase-1 (catalog no. 2225) (1:1000) were purchased from Cell Signaling Technology (Beverly, MA, USA). Monoclonal mouse anti-ASC (sc-271054) (1:500) and polyclonal rabbit anti-IL-1β (sc-7884) (1:500) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Monoclonal mouse anti-NLRP3 (ALX-804-818) (1:500) was purchased from Enzo Life Sciences (Shanghai, China) to detect endogenous NLRP3 in THP1 cells by immunofluorescence microscopy. Lipofectamine 2000, normal rabbit immunoglobulin G (IgG), and normal mouse IgG were purchased from Invitrogen Corporation (Carlsbad, CA, USA).

Lipopolysaccharide (LPS), adenosine triphosphate (ATP), Nigericin, and ProLong™ Diamond Artifade Mountant with Dapi were purchased from InvivoGene Biotech Co., Ltd. (San Diego, CA, USA). PMA was sourced from Sigma-Aldrich (St. Louis, MO, USA). RPMI-1640, DMEM, and FBS were procured from Gibco (Grand Island, NY, USA).
The rabbit anti-GFP-Tag antibody (SAB4301138) and mouse anti-HA-Tag antibody (H9658), along with EZview™ Red anti-HA beads (E6779) and anti-FLAG^® M2 beads (A2220), were obtained from Sigma (St Louis, MO, USA). Monoclonal rabbit anti-NLRP3 (D2P5E), rabbit anti-DYKDDDDK/FLAG Tag antibody (14793), monoclonal rabbit anti-IL-1β (D3U3E), and monoclonal rabbit anti-caspase-1 (2225) were obtained from Cell Signaling Technology (Beverly, MA, USA). Monoclonal mouse anti-ASC (sc-271054) (1:500) and polyclonal rabbit anti-IL-1β (sc-7884) (1:500) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA).