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2 protocols using p hydroxybenzaldehyde

1

Enzymatic Assays for Aromatic Aldehydes

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Vanillin (4-​hydroxy-​3-​methoxybenzaldehyde), vanillic acid (4-hydroxy-3-methoxybenzoic acid), ferulic acid (4-hydroxy-3-methoxycinnamic acid), isoVanillin (3-​hydroxy-​4-​methoxybenzaldehyde), protocatechualdehyde (3,​4-​dihydroxybenzaldehyde), benzaldehyde, p-hydroxybenzaldehyde, m-anisaldehyde (3-​methoxybenzaldehyde), veratraldehyde (3,​4-​dimethoxybenzaldehyde), syringaldehyde (4-​hydroxy-​3,​5-​dimethoxybenzaldehyde), and salicylaldehyde (2-​hydroxybenzaldehyde) were purchased from Wako Pure Chemicals (Osaka, Japan). NAD(P)+ and lactate dehydrogenase from the rabbit muscle were purchased from Oriental Yeast Co. Ltd. (Osaka, Japan). Oligonucleotides were purchased from Life Technologies Japan Ltd. (Tokyo, Japan).
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2

Antioxidant Potential of Sugarcane Compounds

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The following reagents were from commercial sources. Gallic acid monohydrate, 2,2 -azobis (2-amidinopropane) dihydrochloride (AAPH) , 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (trolox) , 2,2 -azinobis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) , and phydroxybenzaldehyde were purchased from Wako Pure Chemical Industries (Osaka, Japan) . Folin-Ciocalteu reagent, sodium carbonate, and p-hydroxyacetophenone were purchased from Nacalai Tesque (Kyoto, Japan) . Fluorescein sodium salt and 1,1-diphenyl-2-picrylhydrazyl (DPPH) were purchased from Sigma-Aldrich (St. Louis, MO., U.S.A.) . p-Coumaric acid was purchased from Tokyo Chemical Industries (Tokyo, Japan) . Schaftoside and isoschaftoside were isolated from sugarcane products and identified based on NMR and UV-Vis spectra previously 8) . All other reagents were from Wako Pure Chemical Industries.
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