Alexa 647 donkey anti rabbit secondary antibodies

Manufactured by Jackson ImmunoResearch

Alexa Fluor 647 donkey anti-rabbit secondary antibodies are fluorescently-labeled secondary antibodies used to detect the presence of rabbit primary antibodies in various immunoassays and imaging applications. These antibodies are conjugated to the Alexa Fluor 647 dye, which has excitation and emission spectra suitable for detection in the far-red/near-infrared range.

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Lab products found in correlation

Alexa488 donkey anti mouse, by Jackson ImmunoResearch (1 mentions) Eclipse ti2 microscope, by National Instruments (1 mentions) Cy3 conjugated donkey anti goat, by Jackson ImmunoResearch (1 mentions) Clone 6e10, by BioLegend (1 mentions) Anti gfap rabbit igg, by Agilent Technologies (1 mentions) Ab27957, by Abcam (1 mentions) M0851, by Agilent Technologies (1 mentions) Ab10558, by Agilent Technologies (1 mentions) Ab13970, by Abcam (1 mentions)

2 protocols using alexa 647 donkey anti rabbit secondary antibodies

Immunohistochemical Profiling of Neuroinflammation

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For immunohistochemistry, brain cryosections were permeabilized with 0.5% Triton X-100 in PBS for 10 min. Nonspecific binding sites were blocked with 3% ovalbumin in PBS solution for 1 h at 37 °C. For labeling reactive astrocytes, microglia and amyloid plaques, brain sections were immunolabeled with primary antibodies anti-GFAP rabbit IgG (Dako, Cat#Z033429-2, 1:500), anti-Iba-1 goat IgG (Novus, Cat#NB100-1028, 1:500), anti-Aβ42, Biolegend, Clone 6E10, Cat#803001, 1:500 or Cat#800701, Clone 4G8, 1:200) and after washing with PBS, Alexa 488 donkey anti-mouse (Jackson ImmunoResearch, Cat#715-546-150, 1:250). Cy3-conjugated donkey anti-goat (Jackson ImmunoResearch, Cat#705-166-147, 1:250) and Alexa 647 donkey anti-rabbit secondary antibodies (Jackson ImmunoResearch, Cat#711-606-152, 1:250). Slices were embedded with Fluoroshield containing DAPI. Fluorescence analysis was performed with a Nikon Ti2 Eclipse microscope equipped with NIS Elements software as described.

Zhu Z., Zhang L., Elsherbini A., Crivelli S.M., Tripathi P., Harper C., Quadri Z., Spassieva S.D, & Bieberich E. (2023). The S1P receptor 1 antagonist Ponesimod reduces TLR4-induced neuroinflammation and increases Aβ clearance in 5XFAD mice. eBioMedicine, 94, 104713.

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Histopathological Lung Tissue Analysis

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Tissue samples of control lungs and tumor-bearing lungs were routinely fixed with formalin and embedded in paraffin. Tissues were then serially sectioned in 4 to 7 μM distances and transferred to slides for hematoxylin and eosin staining.
For immuno-fluorescent staining, the following antibodies were used: GFP was amplified by chicken polyclonal antibodies to GFP (ab13970; Abcam, Cambridge, England, United Kingdom) followed by Alexa 488 donkey anti-chicken secondary antibodies (Jackson, 703-545-155). Rabbit pAb to S100A4 (ab27957; Abcam) followed by Alexa 647 donkey anti-rabbit secondary antibodies (Jackson, 711-605-152; JAX Mice, Clinical & Research Services, Bar Harbor, ME) and PE-conjugated anti-mCD45 (clone 30-F11, Biolegend, San Diego, CA). For immunohistochemistry, slides were incubated with anti-CD45 (Abcam, ab10558) or anti-aSMA (DAKO M0851). 3-Amino-9-ethylcarbazole was used for color development and sections were counterstained with hematoxylin.

Weiss I.D., Ella E., Dominsky O., Smith Y., Abraham M., Wald H., Shlomai Z., Zamir G., Feigelson S.W., Shezen E., Bar-Shai A., Alon R., Izhar U., Peled A., Shapira O.M, & Wald O. (2015). In the hunt for therapeutic targets: mimicking the growth, metastasis, and stromal associations of early-stage lung cancer using a novel orthotopic animal model. Journal of thoracic oncology : official publication of the International Association for the Study of Lung Cancer, 10(1).

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