Dulbecco’s Modified Eagle’s Medium (DMEM), Ham’s nutrient mixture F-12, and fetal bovine serum (FBS) were purchased from Life Technologies (Gibco BRL, Grand Island, NY, USA). Palmitate and 4-(2-aminoethyl)benzenesulfonyl fluoride hydrochloride (AEBSF) were obtained from Sigma-Aldrich (St. Louis, MO, USA). The Palmitate was prepared as described previously [30 (link)]. Thapsigargin and GSK2606414 were purchased from Millipore (Billerica, MA, USA). p-IRE antibody (ab48187), ATF6 antibody (ab11909), PRMT1 antibody (ab3768), and PRMT4 antibody (ab110024) were purchased from Abcam (Cambridge, UK). caspase-3 antibody (#9662), p-eIF2α antibody (#9721), and CHOP antibody (#2895) were purchased from Cell Signaling Technology (Beverly, MA, USA). ASYM24 antibody (#07-414) was obtained from Millipore. β-actin antibody (sc-1616) and p-PERK antibody (sc-32577-R) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). All reagents were of the highest purity commercially available.
Asym24 antibody
Manufactured by Merck Group
ASYM24 is a monoclonal antibody designed for use in laboratory research applications. It is a tool for the detection and quantification of target analytes. The core function of ASYM24 is to specifically bind to and recognize its target, enabling researchers to study the presence, distribution, or interactions of the target molecule.
Automatically generated - may contain errors
Lab products found in correlation
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions)
Ham s f 12 nutrient mixture, by Thermo Fisher Scientific (1 mentions)
Caspase 3 antibody 9662, by Cell Signaling Technology (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Thapsigargin, by Merck Group (1 mentions)
Gsk2606414, by Merck Group (1 mentions)
Palmitate, by Merck Group (1 mentions)
4 2 aminoethyl benzenesulfonyl fluoride hydrochloride aebsf, by Merck Group (1 mentions)
β actin antibody sc 1616, by Santa Cruz Biotechnology (1 mentions)
2 protocols using asym24 antibody
1
Palmitate-induced ER stress signaling
2
GFP-ALY Protein Purification from 293T Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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We
made a stable cell
line from 293T cells expressing GFP-ALY growing in DMEM medium plus
10% fetal bovine serum. With this cell line, we made whole cell extract
with H lysis buffer (150 mM NaCl, 20 mM Tris, pH 7.8, 0.5 mM ETDA,
0.2 mM PMSF, 1 mM NaF, 1 mM sodium orthovanadate, 1% Np-40, 1 mM DTT).
The cells in H lysis buffer were incubated on ice for 30 min before
being cleaned by spinning at 12000g for 20 min. Conjugated
GFP antibody beads (Allele Biotech Inc., San Diego, CA) were used
to pull down GFP fusion protein. The samples were dissolved in SDS–PAGE
gels and detected by ASYM24 antibody (Millipore) in Western blot assay
(chemiluminescence reagents).
made a stable cell
line from 293T cells expressing GFP-ALY growing in DMEM medium plus
10% fetal bovine serum. With this cell line, we made whole cell extract
with H lysis buffer (150 mM NaCl, 20 mM Tris, pH 7.8, 0.5 mM ETDA,
0.2 mM PMSF, 1 mM NaF, 1 mM sodium orthovanadate, 1% Np-40, 1 mM DTT).
The cells in H lysis buffer were incubated on ice for 30 min before
being cleaned by spinning at 12000g for 20 min. Conjugated
GFP antibody beads (Allele Biotech Inc., San Diego, CA) were used
to pull down GFP fusion protein. The samples were dissolved in SDS–PAGE
gels and detected by ASYM24 antibody (Millipore) in Western blot assay
(chemiluminescence reagents).
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