Tata box binding protein tbp

Cobalt protoporphyrin (CoPP) was purchased from Enzo Life Sciences, Inc. (USA). Actinomycin D (AD), cycloheximide (CHX), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), and LTA from Staphylococcus aureus were purchased from Sigma-Aldrich Co. (USA). LY294002, PD98059, SP600125 and SB203580 were obtained from A.G. Scientific (USA). The antibodies for iNOS, HO-1, Nrf2, Akt, p38, TATA-box binding protein (TBP) and histone deacetylases (HDAC) were purchased from Santa Cruz Biotechnology (USA), the antibodies for phospho-Akt and phospho-p-38 were purchased from Cell Signaling Technology (USA), and the anti-tubulin antibody was purchased from Bio Genex (USA). The dry shoot systems of D. moniliforme Sw. were obtained and extracted as described previously [30] . In brief, the dry shoot systems (300 g) were extracted with distilled water at 100ºC for 4 h. The extract was filtered through a 0.45-μm filter, freeze-dried (yield: 8 g), and stored at 4ºC. The dried extract was dissolved in phosphate-buffered saline (PBS) and filtered through a 0.22 μm-filter before use.