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Chicken anti rabbit alexafluor 488

Manufactured by Thermo Fisher Scientific

Chicken anti-rabbit AlexaFluor 488 is a secondary antibody labeled with the AlexaFluor 488 fluorescent dye. It is designed to detect and visualize rabbit primary antibodies in various immunoassay applications.

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2 protocols using chicken anti rabbit alexafluor 488

1

Immunofluorescence Antibody Labeling Protocol

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The following primary antibodies were used for immunofluorescence: polyclonal rabbit GFP (Invitrogen/Thermo Fisher Cat# A11122, RRID:AB_221569, 1/500), mouse monoclonal anti-Dlg (Developmental Studies Hybridoma Bank Cat# 4F3, RRID:AB_528203, 1/100), rat monoclonal mCherry (Invitrogen/Thermo Fisher Cat# M11217, RRID:AB_2536611, 1/500), and mouse monoclonal dCsp (Developmental Studies Hybridoma Bank Cat# Ab49, RRID:AB_2307340, 1/100). Secondary antibodies were obtained from Invitrogen and were used at a final concentration of 1/500: chicken anti-rabbit AlexaFluor 488 (Invitrogen/Thermo-Fisher, Cat# A-21441, RRID:AB_2535859), goat anti-mouse AlexaFluor 568 (Invitrogen/Thermo-Fisher, Cat# A11004, RRID:AB_2534072), goat anti-Rat AlexaFluor 568 (Invitrogen/Thermo-Fisher, Cat# A-11077, RRID:AB_141874), and chicken anti-mouse AlexaFluor 568 (Invitrogen/Thermo-Fisher, Cat# A21200, RRID:AB_2535786). Images were captured with Zen Software (Blue Version 2.3 pro HWL, Zeiss).
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2

Immunofluorescence Antibody Labeling Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following primary antibodies were used for immunofluorescence: polyclonal rabbit GFP (Invitrogen/Thermo Fisher Cat# A11122, RRID:AB_221569, 1/500), mouse monoclonal anti-Dlg (Developmental Studies Hybridoma Bank Cat# 4F3, RRID:AB_528203, 1/100), rat monoclonal mCherry (Invitrogen/Thermo Fisher Cat# M11217, RRID:AB_2536611, 1/500), and mouse monoclonal dCsp (Developmental Studies Hybridoma Bank Cat# Ab49, RRID:AB_2307340, 1/100). Secondary antibodies were obtained from Invitrogen and were used at a final concentration of 1/500: chicken anti-rabbit AlexaFluor 488 (Invitrogen/Thermo-Fisher, Cat# A-21441, RRID:AB_2535859), goat anti-mouse AlexaFluor 568 (Invitrogen/Thermo-Fisher, Cat# A11004, RRID:AB_2534072), goat anti-Rat AlexaFluor 568 (Invitrogen/Thermo-Fisher, Cat# A-11077, RRID:AB_141874), and chicken anti-mouse AlexaFluor 568 (Invitrogen/Thermo-Fisher, Cat# A21200, RRID:AB_2535786). Images were captured with Zen Software (Blue Version 2.3 pro HWL, Zeiss).
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