Middlebrook 7h10 solid agar

The colonies were sub-cultured on Middlebrook 7H10 solid agar (Becton Dickinson, Le Pont de Claix, France). Matrix-assisted laser desorption ionization-time of flight-mass spectrometry (MALDI-TOF-MS) profile did not match any of the profiles entered in the Bruker database (version December, 2015). Optical microscopy was performed using a Leica DM 2500 microscope (Leica, Wetzlar, Germany) after Gram staining and Ziehl-Neelsen staining and pictures were taken using a Nikon Digital Sight DS-U1 Camera System (Nikon, Tokyo, Japan). For electron microscopy, 10 µL of a bacterial suspension in PBS were deposited on a 400-mesh Formvar carbon-coated copper grid (Euromedex, Strasbourg, France) for 10 minutes after the glow discharge. The sample was contrasted using a 5% ammonium molybdate solution. Images were collected using a Tecnai G2 (FEI, Munich, Germany), operating at 200 keV and bacteria were measured using Image J software, version 1.

Growth temperature (ranging from 28 °C to 45 °C) was observed after sub-culture on Middlebrook 7H10 solid agar (Becton Dickinson). Ability to grow on 0% to 10% NaCl was determined by supplementing the Middlebrook 7H10 solid medium with 0–10% NaCl. Carbon source utilization and enzyme activities were determined by inoculating a suspension of 6 McFarland on an Api Coryne strip and Api Zym (bioMérieux, Craponne, France) according to the manufacturer’s instructions with an incubation time of 24 hours. Tween 80 hydrolysis test was performed according to the method of Wayne^{21 (link)}. Niacin accumulation was tested using BBL ™Taxo ™TB Niacin test strips (Becton Dickinson) following the manufacturer instructions. Pigment production in the dark and catalase activity were determined according to the standard procedures²² .