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Alexa fluor 700 conjugated anti mouse ly6g 1a8

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Alexa Fluor 700-conjugated anti-mouse-Ly6G (1A8) is a fluorescently-labeled antibody that binds to the Ly6G antigen expressed on mouse neutrophils. It can be used for the detection and quantification of neutrophils in various applications such as flow cytometry and immunohistochemistry.

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Lab products found in correlation

Pe conjugated anti mouse mertk, by Thermo Fisher Scientific (2 mentions) Anti mouse gr 1 rb, by Thermo Fisher Scientific (2 mentions) Anti streptavidin, by Thermo Fisher Scientific (2 mentions) Biotin conjugated anti mouse cd64 x54 5 7.1, by BioLegend (2 mentions) Pe cy7 conjugated anti mouse ccr2 sa203g11, by BioLegend (2 mentions) Lsrii flow cytometer, by BD (2 mentions)

2 protocols using alexa fluor 700 conjugated anti mouse ly6g 1a8

1

Tumor Immune Cell Profiling

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Tumor tissues were harvested and digested with collagenase B. Red blood cells were removed by ACK lysis buffer. Fc receptors were blocked with 3% BSA in PBS containing 2.4G2 antibody (anti-mouse CD16/CD32; BD Pharmingin), followed by staining with the antibodies below. Phycoerythrin (PE)–conjugated anti-mouse-MerTK (DSSMMER), anti-mouse-Gr-1 (RB6–8C5), allophycocyanin (APC)–conjugated anti-mouse-CD206 (MR6F3), anti-mouse-CD8 (53–6.7), PerCP-eFluor 710–conjugated anti-mouse-CD163 (TNKUPJ), PerCP-Cyanine (Cy) 5.5 conjugated anti-mouse-Ly6C (HK1.4), PE-Cy5-conjugated anti-mouse MHC-II (I-A/I-E, M5/114.15.2), PE-Cy7 conjugated anti-mouse-CD4 (GK1.5), and anti-streptavidin antibodies were purchased from Life Technologies (Grand Island, NY). V500-conjugated anti-mouse-CD45 (30-F11), BV605-conjugated anti-mouse-F4/80 (T45–2342), APC-Cy7–conjugated anti-mouse-CD11b (M1/70), anti-mouse-CD3 (145–2C11), and Alexa Fluor 700-conjugated anti-mouse-Ly6G (1A8) antibodies were purchased from BD Bioscience (San Jose, CA). Biotin-conjugated anti-mouse-CD64 (x54–5/7.1) and PE-Cy7-conjugated anti-mouse-CCR2 (SA203G11) antibodies were purchased from BioLegend (San Diego, CA). Data were collected with LSR-II flow cytometer (Becton Dickinson) and analyzed with FlowJo software (version 8.5.2; TreeStar, Ashland, OR).
Wang Y., Goliwas K.F., Severino P.E., Hough K., Van Vessem D., Wang H., Tousif S., Koomullil R.P., Frost A.R., Ponnazhagen S., Berry J.L, & Deshane J.S. (2020). Mechanical Strain Induces Phenotypic Changes in Breast Cancer Cells and Promotes Immunosuppression in the Tumor Microenvironment. Laboratory investigation; a journal of technical methods and pathology, 100(12), 1503-1516.
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2

Tumor Immune Cell Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Tumor tissues were harvested and digested with collagenase B. Red blood cells were removed by ACK lysis buffer. Fc receptors were blocked with 3% BSA in PBS containing 2.4G2 antibody (anti-mouse CD16/CD32; BD Pharmingin), followed by staining with the antibodies below. Phycoerythrin (PE)–conjugated anti-mouse-MerTK (DSSMMER), anti-mouse-Gr-1 (RB6–8C5), allophycocyanin (APC)–conjugated anti-mouse-CD206 (MR6F3), anti-mouse-CD8 (53–6.7), PerCP-eFluor 710–conjugated anti-mouse-CD163 (TNKUPJ), PerCP-Cyanine (Cy) 5.5 conjugated anti-mouse-Ly6C (HK1.4), PE-Cy5-conjugated anti-mouse MHC-II (I-A/I-E, M5/114.15.2), PE-Cy7 conjugated anti-mouse-CD4 (GK1.5), and anti-streptavidin antibodies were purchased from Life Technologies (Grand Island, NY). V500-conjugated anti-mouse-CD45 (30-F11), BV605-conjugated anti-mouse-F4/80 (T45–2342), APC-Cy7–conjugated anti-mouse-CD11b (M1/70), anti-mouse-CD3 (145–2C11), and Alexa Fluor 700-conjugated anti-mouse-Ly6G (1A8) antibodies were purchased from BD Bioscience (San Jose, CA). Biotin-conjugated anti-mouse-CD64 (x54–5/7.1) and PE-Cy7-conjugated anti-mouse-CCR2 (SA203G11) antibodies were purchased from BioLegend (San Diego, CA). Data were collected with LSR-II flow cytometer (Becton Dickinson) and analyzed with FlowJo software (version 8.5.2; TreeStar, Ashland, OR).
Wang Y., Goliwas K.F., Severino P.E., Hough K., Van Vessem D., Wang H., Tousif S., Koomullil R.P., Frost A.R., Ponnazhagen S., Berry J.L, & Deshane J.S. (2020). Mechanical Strain Induces Phenotypic Changes in Breast Cancer Cells and Promotes Immunosuppression in the Tumor Microenvironment. Laboratory investigation; a journal of technical methods and pathology, 100(12), 1503-1516.
+ Open protocol
+ Expand

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