Single-cell suspensions were obtained from peripheral blood, labelled with fluorescent antibodies, and filtered through 40-μm cell strainers. All samples were analysed on a C6 instrument (BD Biosciences) and analysed with FlowJo software. The antibodies used for staining cells were as follows: FITC anti-mouse CD11b (#101205, Biolegend, USA), PE anti-mouse Gr-1 (#108407, Biolegend, USA), and APC anti-mouse CXCR2 (#149311, Biolegend, USA). Mouse neutrophils were defined as cells expressing both Gr-1 and CD11b.
Pe anti mouse gr 1
Manufactured by BioLegend
Sourced in United States
PE anti-mouse Gr-1 is a fluorochrome-conjugated antibody that specifically binds to the Gr-1 antigen expressed on the surface of mouse granulocytes, including neutrophils, eosinophils, and some monocytes. It can be used for the identification and enumeration of these cell populations in flow cytometric analysis.
Automatically generated - may contain errors
Lab products found in correlation
Apc anti mouse cxcr2, by BioLegend (1 mentions)
C6 instrument, by BD (1 mentions)
Fitc anti mouse cd11b, by BioLegend (1 mentions)
Mouse pe selection kit, by STEMCELL (1 mentions)
Macs cell separation system, by Miltenyi Biotec (1 mentions)
Cellquest software, by BD (1 mentions)
Facscan, by BD (1 mentions)
3 protocols using pe anti mouse gr 1
1
Identifying Mouse Neutrophils by Flow Cytometry
2
Gr1+ cell migration assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell separation was conducted using an MACS cell-separation system (Miltenyi Biotec) using PE anti-mouse Gr1 (catalogue #108407; Biolegend) and a mouse PE selection kit (catalogue #18554; STEMCELL Technologies).
In Vitro Chemotaxis Assay Cell migration was detected using a transwell system. Briefly, cells were incubated for 3 hr at 37 C with specified chemokines or a soluble receptor (CCR5-Ig), and cell counts were determined by FACS. The migration index represents the ratio of cells with supplements compared to the non-treated control well.
In Vitro Chemotaxis Assay Cell migration was detected using a transwell system. Briefly, cells were incubated for 3 hr at 37 C with specified chemokines or a soluble receptor (CCR5-Ig), and cell counts were determined by FACS. The migration index represents the ratio of cells with supplements compared to the non-treated control well.
3
Multiparametric Flow Cytometry Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells isolated from peripheral blood were incubated with fluorescein isothiocyanate (FITC) anti-mouse CD11b antibody (1:80; R&D Systems, USA), PE anti-mouse Gr-1 (BioLegend; 1:80), PE anti-mouse β 3 (BioLegend; 1:150), PE anti-mouse C-X-C chemokine receptor 4 (CXCR4) (BioLegend; 1:80), or PE anti-mouse VEGFR2 (1:80; BioLegend) following a published protocol. 33 The cells were subjected to flow cytometer on a FACScan (BD Bioscience, CA, USA), and data were analyzed with Cell Quest Software (version 3.8, BD).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!