All antibodies for flow cytometry were purchased from Biolegend. These studies used the following antibodies: FITC-CD3 (clone UCHT1) FITC-CD19 (clone HIB19) and FITC-CD56 (clone HCD56). Human TruStain FcX was also purchased from Biolegend. The DNA+Protein oligo-conjugated antibodies were produced and provided by Biolegend as part of a collaboration with Mission Bio, Inc. The antibodies in the conjugate pool were the following: CD3 (clone SK7), CD11b (clone RCRF44), CD19 (clone HIB19), CD34 (clone 581), CD38 (clone HIT2), CD45RA (clone HI100), and CD90 (clone 5E10). Antibody conjugates were pooled in equimolar ratios. All Tapestri related reagents were included as part of a Custom Single Cell DNA sequencing kit purchased from Mission Bio, Inc. The Custom amplicon panel used in these studies covers 109 amplicons over 31 genes previously found to be frequently mutated in human myelodysplastic syndromes (MDS), myeloproliferative neoplasms (MPN), and acute myeloid leukemia (AML) (Supplementary Table 1 )27 –29 .
Dna protein oligo conjugated antibodies
Manufactured by BioLegend
DNA+Protein oligo-conjugated antibodies are a type of lab equipment used for various biomedical research applications. These antibodies are conjugated with both DNA and protein oligonucleotides, allowing for multiplex detection and analysis. The core function of this product is to facilitate the simultaneous identification and quantification of multiple target molecules within a sample.
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Lab products found in correlation
2 protocols using dna protein oligo conjugated antibodies
1
Targeted Single-Cell DNA Sequencing of Hematological Malignancies
2
Targeted Single-Cell DNA Sequencing of Hematological Malignancies
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All antibodies for flow cytometry were purchased from Biolegend. These studies used the following antibodies: FITC-CD3 (clone UCHT1) FITC-CD19 (clone HIB19) and FITC-CD56 (clone HCD56). Human TruStain FcX was also purchased from Biolegend. The DNA+Protein oligo-conjugated antibodies were produced and provided by Biolegend as part of a collaboration with Mission Bio, Inc. The antibodies in the conjugate pool were the following: CD3 (clone SK7), CD11b (clone RCRF44), CD19 (clone HIB19), CD34 (clone 581), CD38 (clone HIT2), CD45RA (clone HI100), and CD90 (clone 5E10). Antibody conjugates were pooled in equimolar ratios. All Tapestri related reagents were included as part of a Custom Single Cell DNA sequencing kit purchased from Mission Bio, Inc. The Custom amplicon panel used in these studies covers 109 amplicons over 31 genes previously found to be frequently mutated in human myelodysplastic syndromes (MDS), myeloproliferative neoplasms (MPN), and acute myeloid leukemia (AML) (Supplementary Table 1 )27 –29 .
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