Peritoneal cell pellets collected from the peritoneal fluid were suspended in PBS and stained with the following antibodies: anti-human CD14 -PerCy5.5 (BioLegend,325,621), anti-human CD45 APC-Cy7 (BioLegend, 368,515) and anti-human GPR91/SUCNR1 FITC (Alomone, ASR-090-F). After staining for half an hour, the cells were washed and prepared for FCM (Beckman Coulter). Data were analyzed using FlowJo (v10) software.
Flow cytometry was also performed to analyze the expression of CD80, CD86, CD163, CD206 and MCT1 in macrophages in vivo or THP-1 cells in vitro, as well as SUCNR1 levels in hESCs. The FCM antibodies used were as follows: anti-human CD86 Percp/cy5.5 (BioLegend, 305,419); anti-human/mouse MCT1 PE (R&D, FAB8275P); anti-human/Mouse GPR91/SUCNR1 FITC (Alomone, ASR-090-F); anti-mouse CD45 percp (BioLegend, 103,129); anti-human/Mouse GPR91/SUCNR1 FITC (Alomone, ASR-090-F); anti-mouse CD80 PE (BioLegend, 104,707), anti-mouse CD86 ALexa fluor 700 (BioLegend, 105,024); anti-mouse CD163 BV421 (BioLegend, 155,309); anti-mouse CD206 BV605 (BioLegend, 141,721), anti-mouse CD11b PCy 7(BioLegend, 101,215); anti-mouse F4/80 APC (BioLegend, 123,116).
Tian Q., Ruan J., Wang Y., Xiao Y., Cheng Q., Chen Y., Li M., Chang K, & Yi X. (2024). Extracellular succinate derived from ectopic milieu drives adhesion and implantation growth of ectopic endometrial stromal cells via the SUCNR1 signal in endometriosis. Cell Communication and Signaling : CCS, 22, 82.
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