RNA-seq libraries were prepared using the NEBnext RNA sequencing kit (E7530) according to the manfacturer’s instructions with the following parameters: 1.) rRNA depletion was performed only on total RNA samples using the NEB rRNA depletion kit (E6310). 2.) RNA fragmentation was performed for 10 minutes. Used barcodes are listed in Suppl. Table 1 , all fastq files were uploaded to GEO after de-multiplexing.
Nebnext rna sequencing kit
Manufactured by New England Biolabs
The NEBnext RNA sequencing kit is a library preparation kit designed for next-generation sequencing of RNA samples. The kit provides the necessary reagents and protocols to convert RNA into a sequencing-ready library.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using nebnext rna sequencing kit
1
RNA-seq Library Preparation Protocol
2
RNA-seq Library Preparation Protocol
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