Salivary cortisol, a commonly used measure of acute cortisol, was collected.
Cotton salivettes were purchased from Sarstedt, Inc. and were stored at room
temperature prior to the study. Participants chewed on Cotton salivettes for
45 s at each time of measurement. Six salivary cortisol samples were
collected over the course of the in-laboratory study. After collection, the
samples were stored at −20°C. Briefly before assaying, the samples were
thawed and spun at 3,000 rpm for 4 min to obtain samples with low viscosity.
One thousand microliters of clear saliva was removed for duplicate analysis
of cortisol levels using a time-resolved immunoassay with fluorescence
detection. The assay process has been described previously (Dressendörfer et al.,
1992 ; Kirschbaum & Hellhammer, 1989 (link)). Cortisol is reported in
nanomoles/liter (nmol/L).
Cotton salivettes were purchased from Sarstedt, Inc. and were stored at room
temperature prior to the study. Participants chewed on Cotton salivettes for
45 s at each time of measurement. Six salivary cortisol samples were
collected over the course of the in-laboratory study. After collection, the
samples were stored at −20°C. Briefly before assaying, the samples were
thawed and spun at 3,000 rpm for 4 min to obtain samples with low viscosity.
One thousand microliters of clear saliva was removed for duplicate analysis
of cortisol levels using a time-resolved immunoassay with fluorescence
detection. The assay process has been described previously (
1992
nanomoles/liter (nmol/L).