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Ultrapure 10x tae buffer

Manufactured by Thermo Fisher Scientific

Ultrapure 10X TAE buffer is a concentrated solution used as a stock buffer for gel electrophoresis. It provides the necessary ionic environment to facilitate the separation and migration of DNA, RNA, or protein samples during electrophoresis procedures.

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2 protocols using ultrapure 10x tae buffer

1

Oxidative DNA Damage Quantification

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ROS indicator probes APF and HPF, plasmid DNA (pBR322), ethidium bromide, ultrapure 10X TAE buffer and 1 kb plus DNA ladder were purchased from Invitrogen (Life Technologies, Carlsbad, CA). Sodium hypochlorite, silibinin, quercetin, L-methionine, agarose (ultrapure) and calf thymus DNA were purchased from Sigma-Aldrich (St. Louis, MO). Hypochlorite concentration was documented spectrophotometrically in 10 mM NaOH at pH 12 using molar coefficient at 292 nm (ε292 = 350 M−1 cm−1). Dulbecco’s phosphate buffered saline (DPBS ×1, 21-031-CV) without calcium and magnesium was purchased from Mediatech Inc. (Manassas, VA). Commercially available SDG (com) was purchased from Chromadex (Irvine, CA) and synthetic SDGs (SDG (S,S) and SDG (R,R)) were synthesized by our group [39 (link)].
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2

Oxidative DNA Damage Quantification

Check if the same lab product or an alternative is used in the 5 most similar protocols
ROS indicator probes APF and HPF, plasmid DNA (pBR322), ethidium bromide, ultrapure 10X TAE buffer and 1 kb plus DNA ladder were purchased from Invitrogen (Life Technologies, Carlsbad, CA). Sodium hypochlorite, silibinin, quercetin, L-methionine, agarose (ultrapure) and calf thymus DNA were purchased from Sigma-Aldrich (St. Louis, MO). Hypochlorite concentration was documented spectrophotometrically in 10 mM NaOH at pH 12 using molar coefficient at 292 nm (ε292 = 350 M−1 cm−1). Dulbecco’s phosphate buffered saline (DPBS ×1, 21-031-CV) without calcium and magnesium was purchased from Mediatech Inc. (Manassas, VA). Commercially available SDG (com) was purchased from Chromadex (Irvine, CA) and synthetic SDGs (SDG (S,S) and SDG (R,R)) were synthesized by our group [39 (link)].
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