Genome editing was carried out in a doxycycline-inducible Cas9 HEK293T cell line, transduced with pCW-Cas9 plasmid (Addgene plasmid #50661 [38 (link)]), courtesy of the Sagi Shapira Lab. HEK293T cells were cultured in OptiMEM (Gibco) supplemented with 5% HyClone Cosmic Calf Serum (Fisher), 1% GlutaMAX (Gibco), 1% NaPyr (Corning), and 1% penicillin/streptomycin (Corning). The cells were passaged and maintained following the standard techniques in 5% CO2 and 95% air.
For replication in HeLa cells, cells were cultured in DMEM (Corning) with 4.5 g/L glucose supplemented with 10% heat-inactivated FBS (Gibco), 1% GlutaMAX (Gibco), 1% NaPyr (Corning), and 1% penicillin/streptomycin (Corning).
For replication in HeLa cells, cells were cultured in DMEM (Corning) with 4.5 g/L glucose supplemented with 10% heat-inactivated FBS (Gibco), 1% GlutaMAX (Gibco), 1% NaPyr (Corning), and 1% penicillin/streptomycin (Corning).