Atropine sulphate

[1,3-bis(4-hydroxyiminomethylpyridinium) propane dichloride] was synthesised in Bosnalijek, Sarajevo, Bosnia and Herzegovina, while HI-6 [(1-(2-hydroxyiminomethylpyridinium)-3-(4-carbamoylpyridinium)-2-oxapropane dichloride)] was synthesised at the University of Defence, Hradec Kralove, Czech Republic (16) . Oximes were kept at room temperature and dissolved in distilled water or atropine immediately before use. Paraoxon (diethyl p-nitrophenyl phosphate) was purchased from Sigma-Aldrich, Steinheim, Germany. The stock solution of 50 mg mL -1 of Paraoxon was prepared in isopropanol. Further dilutions were made in saline, shortly before use. Atropine sulphate was purchased from Kemika, Zagreb, Croatia. The solution of 5 mg mL -1 of atropine was prepared in distilled water.

Oxime K048 (1-(4-hydroxyiminomethylpyridinium)-4-(4-carbamoylpyridinium) butane dibromide) (Mr=460.16 g mol -1 ) was a gift from Dr Kamil Kuča, University of Hradec Kralove, Czech Republic. It was kept at room temperature and dissolved in water or atropine immediately before use. Tabun (ethyl N,N-dimethyl phosphoroamidocyanidate) was purchased from NC Laboratory, Spiez, Switzerland. A stock solution of 5,000 μg mL -1 was prepared in isopropyl alcohol. Further dilutions were made in saline immediately before use. Atropine sulphate (Kemika, Zagreb, Croatia) was dissolved in water. Non-ionic detergent 1 % Triton X-100 was purchased from SERVA Electrophoresis GmbH, Heidelberg, Germany. Acetylthiocholine iodide (ATCh), DTNB (5,5'-dithiobis(2-nitrobenzoic acid)), BW284C51 (1,5-bis(4-allyldimethyl-ammoniumphenyl)-pentane-3-one dibromide), ethopropazine ((10-[2-diethylaminopropyl] phenothiazine)hydrochloride), and chemicals for high performance liquid chromatography (HPLC) [acetonitrile gradient grade LiChrosolv, disodium hydrogen phosphate dihydrate, citric acid monohydrate, ethylenedinitrilo tetraacetic acid disodium salt dihydrate (Na 2 EDTA), 1-octane sulphonic acid sodium salt, perchloric acid (70 %), and phosphoric acid] were purchased from Sigma-Aldrich (St. Louis, MO, USA). The water used for HPLC was double-distilled and deionised HPLC-grade.