Forebrain and liver tissue samples were homogenized with RIPA buffer (Cell Signaling Technology, Danvers, MA) containing a cocktail of protease and phosphatase inhibitors (MilliporeSigma, Burlington, MA). Total protein was extracted from eWAT using the Minute Total Protein Extraction Kit for Adipose Tissues (Invent Biotechnologies, Plymouth, MN) following the manufacturer's instructions. Equal amounts of total protein were separated by an SDS-PAGE gel, transferred to a PVDF membrane (Bio-Rad) and immunoblotted with antibodies: anti-HSC70 (MilliporeSigma, Burlington, MA), anti-actin (MilliporeSigma) and anti-tubulin (Cell Signaling Technology), anti-TN and anti-TX (generated in our lab). Blots were developed with the ECL system (Thermo Fisher Scientific). Band intensities were quantified from digital images by densitometry using ImageJ.
Anti hsc70
Manufactured by Merck Group
Anti-HSC70 is a laboratory product that functions as an antibody targeting the Heat Shock Cognate 70 (HSC70) protein. HSC70 is a constitutively expressed chaperone protein involved in various cellular processes. The Anti-HSC70 product can be used in research applications to investigate the role and regulation of HSC70.
Automatically generated - may contain errors
Lab products found in correlation
Anti tubulin, by Cell Signaling Technology (2 mentions)
Ecl system, by Thermo Fisher Scientific (2 mentions)
Minute total protein extraction kit for adipose tissues, by Invent Biotechnologies (2 mentions)
Ripa buffer, by Cell Signaling Technology (2 mentions)
Anti actin, by Merck Group (2 mentions)
Of protease and phosphatase inhibitors, by Merck Group (2 mentions)
Pvdf membrane, by Bio-Rad (2 mentions)
2 protocols using anti hsc70
1
Protein Extraction and Immunoblotting
2
Protein Extraction and Analysis from Tissues
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Forebrain and liver tissue samples were homogenized with RIPA buffer (Cell Signaling Technology, Danvers, MA) containing a cocktail of protease and phosphatase inhibitors (MilliporeSigma, Burlington, MA). Total protein was extracted from eWAT using the Minute Total Protein Extraction Kit for Adipose Tissues (Invent Biotechnologies, Plymouth, MN) following the manufacturer's instructions. Equal amounts of total protein were separated by an SDS-PAGE gel, transferred to a PVDF membrane (Bio-Rad) and immunoblotted with antibodies: anti-HSC70 (MilliporeSigma, Burlington, MA), anti-actin (MilliporeSigma) and anti-tubulin (Cell Signaling Technology), anti-TN and anti-TX (generated in our lab). Blots were developed with the ECL system (Thermo Fisher Scientific). Band intensities were quantified from digital images by densitometry using ImageJ.
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