Attenuated total reflection infrared (ATR FT-IR) spectra of untreated and HPH treated BSA or WPI dispersions (P = 200 MPa, nP = 1) were measured according to the protocol described by Siddique et al. (2016) by using an FTIR-4100 series spectrophotometer (Jasco Europe Srl, Italy).
The spectra were collected at a resolution of 2 cm -1 in the Amide I region (1700 -1600 cm -1 ) in the double-sided, forward-backward mode and resulted from an average of 64 scans. The spectra of protein dispersions were corrected by subtracting the background noise originating from the air, moisture, and coating materials on reflecting mirrors along the IR radiation path. Nine repetitions from each sample were used for each spectra measurement. The resulting averaged spectra were smoothed with an eleven-point under adaptive-smoothing function to remove the eventual noises and then baseline modification was applied.
The spectra were collected at a resolution of 2 cm -1 in the Amide I region (1700 -1600 cm -1 ) in the double-sided, forward-backward mode and resulted from an average of 64 scans. The spectra of protein dispersions were corrected by subtracting the background noise originating from the air, moisture, and coating materials on reflecting mirrors along the IR radiation path. Nine repetitions from each sample were used for each spectra measurement. The resulting averaged spectra were smoothed with an eleven-point under adaptive-smoothing function to remove the eventual noises and then baseline modification was applied.