The largest database of trusted experimental protocols

3 protocols using human osteoblast like mg 63 cells

1

Fabrication of PLLA/GO Scaffolds by SLS

Check if the same lab product or an alternative is used in the 5 most similar protocols
PLLA powders (Purity: tionigand inherent viscosity: 1.46 dl/g) were provided from Shenzhen Polymtek Biomaterial Co., Ltd. (Shenzhen, China). GO powders (purity > 98%) were obtained from Chengdu Organic Chemicals Co., Ltd. (Chengdu, China). The composition of phosphate-buffered solution (PBS) was NaCl, KCl, Na2HPO2H2O, and KH2PO4, which was provided from Sigma-Aldrich (Beijing, China). SBF was purchased from Qingdao Jieshang Biological Technology Co., Ltd. (Qingdao, China). Human osteoblast-like MG-63 cells were supplied from the American Type Culture Collection (ATCC, Rockville, MD).
Three-dimensional porous PLLA and PLLA/GO scaffolds with 0.3%, 0.6%, 0.9%, and 1.2% GO were prepared by SLS. The powders were evenly laid on the sintering platform and selectively sintered according to the pre-planned scaffold models, and the sintering platform would be lowered to a corresponding height when a layer of powders was sintered and then a layer of powders was applied again, sintering layer by layer, and finally obtaining the required scaffolds. The processing parameters were applied as follows: Spot size of 1.2 mm, laser power of 2.2 W, and laser scanning speed of 100 mm/min.
+ Open protocol
+ Expand
2

Facile Synthesis of Zirconia-Based Biomaterials

Check if the same lab product or an alternative is used in the 5 most similar protocols
AMES Group supplied yttria-stabilized zirconia (Y-TZP) rods. The fine-grained zirconia was stabilized with a 2.5% molar of Y2O3. The material composition and main properties are listed in Table S1. The zirconia discs (diameter of 8.00 mm and thickness of 2.00 ± 0.01 mm) were cut from rods with IsoMet 4000 linear precision saw from Buehler using a diamond cutting disc, polished until mirror grade with diamond polishing discs using Phoenix 4000 polisher machine from Buehler manufacturer. Then, the samples were cleaned in an ultrasonic bath, first with distilled water (3 times, 5 min per time), then with ethanol absolute (3 times, 5 min per time). The samples were stored in a desiccator until use.
Ethylene glycol dimethacrylate (EGDMA, 98%) was purchased from Sigma-Aldrich and used as received. The monomer methyl-DOPA methacrylamide (DOMAm), methyl 3-(3,4-dihydroxyphenyl)-2-(2-methylprop-2-enamido)propanoate, was kindly provided by Symbiose Biomaterials, Belgium.
Human osteoblast-like MG-63 cells were obtained from American Type Culture Collection (ATCC, USA) for the cell viability study, and Dulbecco's phosphate buffered saline (DPBS) was obtained from Gibco (NY, USA). Milli-Q water was used for the sample's stability coating determination.
+ Open protocol
+ Expand
3

Culturing Human Osteoblasts and Mesenchymal Stem Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Human osteoblast-like MG63 cells (American Type Culture Collection, Manassas, VA) were seeded at an initial density of 10,000 cells/cm2 and cultured in Dulbecco’s modification of Eagle’s medium (DMEM, Mediatech, Inc., Manassas, VA) supplemented with 10% fetal bovine serum (Life Technologies, Carlsbad, CA) and 1% penicillin-streptomycin (Life Technologies). Human bone marrow mesenchymal stem cells (MSCs, Lonza, Walkersville, MD) were plated at 5,000 cells/cm2 and cultured in Mesenchymal Stem Cell Growth Medium (Lonza). All cells were cultured at 37°C with 5% CO2 and 100% humidity.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!