Three-dimensional porous PLLA and PLLA/GO scaffolds with 0.3%, 0.6%, 0.9%, and 1.2% GO were prepared by SLS. The powders were evenly laid on the sintering platform and selectively sintered according to the pre-planned scaffold models, and the sintering platform would be lowered to a corresponding height when a layer of powders was sintered and then a layer of powders was applied again, sintering layer by layer, and finally obtaining the required scaffolds. The processing parameters were applied as follows: Spot size of 1.2 mm, laser power of 2.2 W, and laser scanning speed of 100 mm/min.
Human osteoblast like mg 63 cells
The human osteoblast-like MG-63 cells are a cell line derived from a human osteosarcoma. They exhibit characteristics of osteoblast-like cells and are commonly used in research applications involving bone and skeletal tissues.
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3 protocols using human osteoblast like mg 63 cells
Fabrication of PLLA/GO Scaffolds by SLS
Three-dimensional porous PLLA and PLLA/GO scaffolds with 0.3%, 0.6%, 0.9%, and 1.2% GO were prepared by SLS. The powders were evenly laid on the sintering platform and selectively sintered according to the pre-planned scaffold models, and the sintering platform would be lowered to a corresponding height when a layer of powders was sintered and then a layer of powders was applied again, sintering layer by layer, and finally obtaining the required scaffolds. The processing parameters were applied as follows: Spot size of 1.2 mm, laser power of 2.2 W, and laser scanning speed of 100 mm/min.
Facile Synthesis of Zirconia-Based Biomaterials
Ethylene glycol dimethacrylate (EGDMA, 98%) was purchased from Sigma-Aldrich and used as received. The monomer methyl-DOPA methacrylamide (DOMAm), methyl 3-(3,4-dihydroxyphenyl)-2-(2-methylprop-2-enamido)propanoate, was kindly provided by Symbiose Biomaterials, Belgium.
Human osteoblast-like MG-63 cells were obtained from American Type Culture Collection (ATCC, USA) for the cell viability study, and Dulbecco's phosphate buffered saline (DPBS) was obtained from Gibco (NY, USA). Milli-Q water was used for the sample's stability coating determination.
Culturing Human Osteoblasts and Mesenchymal Stem Cells
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