Rabbit anti gap43

Manufactured by Novus Biologicals

Rabbit anti-Gap43 is a primary antibody that binds to the Growth-Associated Protein 43 (GAP43), a membrane-bound protein involved in axonal growth and neural development. This antibody is commonly used in research applications to detect and study the expression and localization of GAP43 in various tissues and cell types.

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Lab products found in correlation

Fluorescence conjugated secondary antibody, by Jackson ImmunoResearch (1 mentions) Mouse anti map2, by Merck Group (1 mentions) Odyssey infrared scanner, by LI COR (1 mentions) Rabbit anti gapdh, by Bioworld Technology (1 mentions) Rabbit anti ptch1, by Abcam (1 mentions) Rabbit anti gli 1, by Bioworld Technology (1 mentions)

Close spelling variants of this product

GAP43 (7 citations)

2 protocols using rabbit anti gap43

Neuronal Marker Immunostaining Protocol

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Third instar larvae or cultured neurons were fixed according to standard protocols. The following antibodies were used: mouse anti-FasII 1D4 antiserum (1:2, Developmental Studies Hybridoma Bank), rabbit anti-Gap43 (1:1000, Novus Biologicals), mouse anti-MAP2 (1:1000, Sigma) rabbit anti-phospho-CaMKII alpha/beta/delta (Thr305) (1:50, Thermo Fisher Scientific), and fluorescence-conjugated secondary antibodies (1:1000, Jackson ImmunoResearch).

Song Y., Li D., Farrelly O., Miles L., Li F., Kim S.E., Lo T.Y., Wang F., Li T., Thompson-Peer K.L., Gong J., Murthy S.E., Coste B., Yakubovich N., Patapoutian A., Xiang Y., Rompolas P., Jan L.Y, & Jan Y.N. (2019). The Mechanosensitive Ion Channel Piezo Inhibits Axon Regeneration. Neuron, 102(2), 373-389.e6.

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Hedgehog Pathway Protein Analysis

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Proteins were isolated from brain tissue and analyzed using the standard SDS-PAGE method. Immunoreactivity was determined by semiquantitative gel density scanning. The following antibodies were used: rabbit anti-Shh (1:1000, Boster), rabbit anti-Ptch-1 (1:500, Abcam), mouse anti-Smo (1:300, Proteintech), rabbit anti-Gli-1 (1:500, Bioworld), rabbit anti-GAP43 (1:5000, NOVUS), and rabbit anti-GAPDH (1:10,000, Bioworld). Membranes were scanned with an Odyssey infrared scanner (LICOR Bioscience, Lincoln, NE, USA) and analyzed with ImageJ software. The relative densitometric values were quantified with respect to GAPDH.

Cao W., Zhang C., Chen R., Wu Q., Xu R., Zhang L, & Zhang X. (2020). A Novel Cerebroprotein Hydrolysate, CH1, Ameliorates Chronic Focal Cerebral Ischemia Injury by Promoting White Matter Integrity via the Shh/Ptch-1/Gli-1 Signaling Pathway. Neuropsychiatric Disease and Treatment, 16, 3209-3224.

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