Cyclic guanosine monophosphate cgmp

Glucose was measured using a FreeStyle Lite® (Abbott) glucometer with accompanying strips, and triglyceride assay reagents were procured from Sigma. Insulin (Humulin R, Lilly) was purchased at a local pharmacy. Insulin ELISA kits were from ALPCO Diagnostics. S-Nitro-N-acetyl-DL-penicillamine (SNAP) and cyclic guanosine monophosphate (cGMP) were procured from Cayman Chemical Company. Sodium chloride, potassium chloride, calcium chloride, magnesium chloride, sodium bicarbonate, potassium phosphate, D-glucose, collagenase, ethylenediaminetetraacetic acid (EDTA), ethylene glycol tetraacetic acid (EGTA), sodium pyrophosphate, sodium orthovanadate, sodium fluoride, okadaic acid, 1% protease inhibitor cocktail, dithiothreitol, magnesium chloride, K-lactobionate, taurine, potassium phosphate, HEPES, digitonin, pyruvate, malic acid, glutamic acid, adenosine diphosphate, succinic acid, oligomycin, carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone, phenylephrine and ACh, trypsin inhibitor, and cytochrome c were procured from Sigma-Aldrich (MO, USA). Mammalian Protein Extraction Reagent (M-PER) was from Thermo Scientific, and Immobilon-P PVDF membrane was from Millipore. Sepiapterin was procured from Sigma-Aldrich.

The lung tissue was isolated and prepared to detect the concentrations of cyclic guanosine monophosphate (cGMP) (Cayman Chemical, Michigan, USA) and superoxide anion and the activity of superoxide dismutation (SOD) using the commercial kits (Solarbio, Beijing, China). Moreover, the expression levels of iNOS, eNOS and phosphorylated eNOS were also detected in the lung tissue using Western blot.