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Axiovert 200 microscope

Manufactured by Teledyne
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Sourced in Azerbaijan

The Axiovert 200 is a high-performance inverted microscope designed for a variety of laboratory applications. It features a stable, ergonomic design and provides excellent optical performance for imaging and observation.

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Lab products found in correlation

Metafluor software, by Universal Imaging (3 mentions) Photometrics cascade 512b ccd camera, by Teledyne (2 mentions) Mitotracker red cmxros, by Thermo Fisher Scientific (2 mentions) Oil immersion objective, by Zeiss (1 mentions) Coolsnap hq, by Teledyne (1 mentions)

Close spelling variants of this product

Axiovert 200 M microscope

3 protocols using axiovert 200 microscope

1

Evaluating Mitochondrial Function in Fibroblasts

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Fibroblasts from the affected sibling harbouring the SLC25A1 variant c.740G > A; p.R247Q and control fibroblasts were grown in DMEM (high glucose). Membrane potential was measured by fluorescence microscopy using the tetramethylrhodamine methyl ester (TMRM) probe [22 (link)], while mitochondrial morphology was investigated using MitoTracker® Red CMXRos. MitoTracker® Red CMXRos and tetramethylrhodamine methyl ester (TMRM) were purchased from Life Technologies.
Fluorescent images of treated cells were acquired and fluorescence intensities were analyzed, as previously described [23 (link)], using a Zeiss Axiovert 200 microscope equipped with a Photometrics Cascade 512B CCD camera (Roper Scientific) and the MetaFluor software (Universal Imaging).
Chaouch A., Porcelli V., Cox D., Edvardson S., Scarcia P., De Grassi A., Pierri C.L., Cossins J., Laval S.H., Griffin H., Müller J.S., Evangelista T., Töpf A., Abicht A., Huebner A., von der Hagen M., Bushby K., Straub V., Horvath R., Elpeleg O., Palace J., Senderek J., Beeson D., Palmieri L, & Lochmüller H. (2014). Mutations in the Mitochondrial Citrate Carrier SLC25A1 are Associated with Impaired Neuromuscular Transmission. Journal of neuromuscular diseases, 1(1), 75-90.
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2

Fluorescence Imaging of Cerulean/Venus Ratio

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The culture medium was replaced with HBSS for fluorescent imaging. The cells were imaged at 25 °C on a Carl Zeiss Axiovert 200 microscope with a cooled CCD camera CoolSNAP HQ (Roper Scientific Inc. Tucson, AZ) controlled by the MetaFluor software (Universal Imaging, West Chester, PA). The exposure time at 440 ± 10.5 nm excitation was 100 ms and 300 ms for Venus and Cerulean, respectively. Fluorescence images were obtained through 480 ± 15 nm and 535 ± 13 nm filters with a 40x oil immersion objective (Carl Zeiss, Jena, Germany). The images in this study were displayed in intensity modulated display (IMD) mode, in which the intensity of the fluorescence is considered to make the images displayed with Cerulean/Venus ratio images.
Ueda Y., Ogiso H., Sato M., Umezawa Y., Okazaki T, & Kobayashi T. (2015). Asymmetrical diacylglycerol dynamics on the cytosolic and lumenal sides of a single endomembrane in living cells. Scientific Reports, 5, 12960.
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3

Evaluating Mitochondrial Function in Fibroblasts

Check if the same lab product or an alternative is used in the 5 most similar protocols
Fibroblasts from the affected sibling harbouring the SLC25A1 variant c.740G > A; p.R247Q and control fibroblasts were grown in DMEM (high glucose). Membrane potential was measured by fluorescence microscopy using the tetramethylrhodamine methyl ester (TMRM) probe [22 (link)], while mitochondrial morphology was investigated using MitoTracker® Red CMXRos. MitoTracker® Red CMXRos and tetramethylrhodamine methyl ester (TMRM) were purchased from Life Technologies.
Fluorescent images of treated cells were acquired and fluorescence intensities were analyzed, as previously described [23 (link)], using a Zeiss Axiovert 200 microscope equipped with a Photometrics Cascade 512B CCD camera (Roper Scientific) and the MetaFluor software (Universal Imaging).
Chaouch A., Porcelli V., Cox D., Edvardson S., Scarcia P., De Grassi A., Pierri C.L., Cossins J., Laval S.H., Griffin H., Müller J.S., Evangelista T., Töpf A., Abicht A., Huebner A., von der Hagen M., Bushby K., Straub V., Horvath R., Elpeleg O., Palace J., Senderek J., Beeson D., Palmieri L, & Lochmüller H. (2014). Mutations in the Mitochondrial Citrate Carrier SLC25A1 are Associated with Impaired Neuromuscular Transmission. Journal of neuromuscular diseases, 1(1), 75-90.
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