Pea seeds (Pisum sativum var. Bajka) were obtained from Company of Horticulture Seeds and Nursery in Ożarów Mazowiecki, Poland. HHL (hippuryl-L-histidyl-L-leucine), pepstatin A, PMSF (phenylmethanesulfonyl fluoride), α-amylase from hog pancreas (50 U/mg, 10080, Sigma), pepsin from porcine gastric mucosa (250 U/mg, P7000, Sigma), pancreatin from porcine pancreas (P7545, Sigma), enalapril, bile extract, and TNBS (2,4,6-trinitrobenzenesulfonic acid) were purchased from Sigma-Aldrich Company, USA, and Amino Acid Standard from Pierce, USA. Any other chemicals were of analytical grade.
α amylase from hog pancreas
Manufactured by Merck Group
Sourced in United States
α-amylase from hog pancreas is an enzyme that hydrolyzes α-1,4-glycosidic bonds in polysaccharides, such as starch and glycogen. It is widely used in various industrial and research applications.
Automatically generated - may contain errors
Lab products found in correlation
α glucosidase from saccharomyces cerevisiae, by Merck Group (3 mentions)
Pepstatin a, by Merck Group (1 mentions)
2 4 6 trinitrobenzenesulfonic acid tnbs, by Merck Group (1 mentions)
Bile extract, by Merck Group (1 mentions)
P7000, by Merck Group (1 mentions)
Pancreatin from porcine pancreas, by Merck Group (1 mentions)
Enalapril, by Merck Group (1 mentions)
Hepg2 cell, by American Type Culture Collection (1 mentions)
3t3 l1 cells, by American Type Culture Collection (1 mentions)
P nitrophenyl α d galactopyranoside p npg, by Merck Group (1 mentions)
Iron 3 chloride, by Merck Group (1 mentions)
Porcine bile extract, by Merck Group (1 mentions)
Crystalline uric acid, by Merck Group (1 mentions)
Abts diammonium salt, by Merck Group (1 mentions)
3 2 pyridyl 5 6 diphenyl 1 2 4 triazine p p disulfonic acid monosodium salt hydrate ferrozine, by Merck Group (1 mentions)
Sodium phosphate monobasic anhydrous, by Daejung Chemicals (1 mentions)
Iron 2 chloride, by Merck Group (1 mentions)
Lipase, by Merck Group (1 mentions)
Trichloroacetic acid, by Daejung Chemicals (1 mentions)
Quercetin, by Merck Group (1 mentions)
5 protocols using α amylase from hog pancreas
1
Pisum sativum Protein Digestion Assay
2
Enzyme Inhibition and Characterization
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OA was purchased from Energy Chemical Company, China. α-glucosidase from Saccharomyces cerevisiae (EC 3.2.1.20), α-amylase from hog pancreas (EC 3.2.1.1), and p-nitrophenyl-α-D-galactopyranoside (p-NPG) were provided by Sigma-Aldrich. Water-soluble starch was obtained from Shanghai Yuanye Biological Technology Co., Ltd. 3T3-L1 cells and HepG2 cells were supplied by ATCC. Other reagents were purchased from commercial suppliers. All the compounds were dissolved in DMSO, and DMSO working concentration in the enzyme inhibition test was 5%. The NMR spectra were recorded on a Bruker AM spectrometer (500 MHz). High-resolution mass spectral analysis (HRMS) was carried out on the Apex II by means of the ESI technique. Melting points were detected on a WRS-2C micro melting point instrument.
3
Enzymatic Assays for Antioxidant Evaluation
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α-Amylase from hog pancreas (CAS No. 9000-90-2), porcine bile extract (CAS No. 8008-63-7), lipase from porcine pancreas (CAS No. 9001-62-1), mucin from porcine stomach Type II (CAS No. 84082-64-4), pepsin from porcine gastric mucosa (CAS No. 9001-75-6), crystalline uric acid (≥99%; CAS No. 69-93-2), 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) diammonium salt (CAS No. 30931-67-0), 2,2-diphenyl-1-picrylhudrazyl (DPPH; CAS No. 1898-66-4), 3-(2-pyridyl)-5,6-diphenyl-1,2,4-triazine-p,p′-disulfonic acid monosodium salt hydrate (ferrozine), iron (II) chloride (CAS No. 7758-94-3), and iron (III) chloride (CAS No. 7705-08-0) were purchased from Sigma-Aldrich (St. Louis, MA, USA). EDTA-2Na dihydrate (CAS No. 6381-92-6), trichloroacetic acid (TCA; CAS No. 76-03-9), sodium phosphate dibasic anhydrous (CAS No. 7558-79-4), and sodium phosphate monobasic anhydrous (CAS No. 7558-80-7) were purchased from Daejung Chemicals (Siheung, Korea). Potassium hexacyanoferrate (III) (CAS No. 13746-66-2) was purchased from Kanto Chemical (Tokyo, Japan) and 2,6-di-tert-butyl-4-methylphenol (99.0%) was purchased from Samchun Pure Chemical (Seoul, Korea).
4
Phytochemical Profiling and Bioactivity Evaluation
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Flavonoid and isoflavonoid compounds, including rutin, quercitrin, quercetin 3-β-D-glucoside, quercetin, isorhamnetin, formononetin, genistein, naringenin, biochanin A, and vitexin, were purchased from Sigma–Aldrich (Schnelldorf, Germany), daidzein was obtained from Fluka (Buchs, Switzerland), luteolin and kaempferol were purchased from Carl Roth (Karlsruhe, Germany), 2,2-difenil-1-picrilhidrazil (DPPH), potassium ferricyanide, sodium carbonate (Na2CO3), dinitrosalicylic acid (DNS), α-amylase from hog pancreas, α-glucosidase from Saccharomyces cerevisiae, and 4-nitrophenyl α-d -glucopyranoside (NPG) were purchased from Sigma–Aldrich, and iron chloride was bought from Fluka. All other used reagents, methanol (Riedel-de Haen), and ethanol (Chemical Company) were of chromatographic or analytical purity; the ultra-pure water was obtained using the distillation apparatus from Evoqua Water Technologies (Pittsburgh, PA, USA).
The medicinal plants were collected from the Cluj county (Romania) natural site, and voucher specimens were stored in the Herbarium of Babes-Bolyai University from Cluj-Napoca (code: 868.786 for Solidago virgaurea L.; code: 622172 for Medicago sativa).
The medicinal plants were collected from the Cluj county (Romania) natural site, and voucher specimens were stored in the Herbarium of Babes-Bolyai University from Cluj-Napoca (code: 868.786 for Solidago virgaurea L.; code: 622172 for Medicago sativa).
5
Enzymatic Assay for Carbohydrate Metabolism
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Reagents and standards as 4-p-nitrophenyl-α-D-glucopyranoside, α-glucosidase from Saccharomyces cerevisiae (CAS number: 9001-42-7), α-amylase from hog pancreas (CAS number: 9000-90-2), dimethyl sulfoxide (DMSO), iodine, potassium iodide, potassium phosphate monobasic, sodium carbonate, sodium chloride, starch, acarbose and orlistat were purchased from Sigma-Aldrich (Milan, Italy). The QuantiChrom™ lipase assay kit was purchased from BioAssay Systems (BioAssay Systems, Hayward, CA, USA).
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