The wet carbonaceous gel was taken out directly from the Teflon container and cut into pieces of desired shape and size. Small pieces of carbonaceous nanofiber hydrogels were soaked successively in ethanol (12 h × 5 times), acidic H2O2 solution (HCl: H2O2: H2O = 2:5:23, v/v, 12 h), and deionized water (12 h × 5 times) at room temperature to remove impurities and TeNWs templates. The purified hydrogel was frozen in liquid nitrogen (−196°C) and freeze-dried in a bulk tray dryer (Labconco Corporation, USA) at a sublimating temperature of −48°C and a pressure of 0.04 mbar. The obtained carbonaceous nanofiber aerogels were transferred into a tubular furnace for pyrolysis under an argon flow. The carbonaceous nanofiber aerogels were heated to 500°C at a heating rate of 2°C/min, kept this temperature for 1 h, then heated to 1450°C at 5°C/min and held at this temperature for 2 h to allow for complete pyrolysis. They were then cooled to 500°C at 5°C/min and finally cooled to room temperature naturally to yield black CNF aerogels.
Bulk tray dryer
Manufactured by Labconco
Sourced in United States
The Bulk Tray Dryer is a laboratory equipment designed for drying various materials in bulk quantities. It provides a controlled environment for the drying process, ensuring consistent and efficient drying results. The core function of the Bulk Tray Dryer is to remove moisture from samples or materials placed on its trays through the application of heat and air flow.
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Lab products found in correlation
5 protocols using bulk tray dryer
1
Synthesis of Carbonaceous Nanofiber Aerogels
2
Synthesis of Cerium Oxide Nanowires
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All the chemicals were of analytical grade and used without further purification. Firstly, 2 mmol (0.6345 g) cerium acetate was dissolved in a flask with 80 ml of a water/ethanol mixed solution with a 1 : 1 volume ratio. Then, the flask was placed in an oil bath and 20 ml of 30% NH3·H2O was added when the temperature increased to 140 °C. The reaction mixture was refluxed at 140 °C for 12 h under stirring. After the solution was cooled to room temperature, the resulting products were centrifuged and washed with ethanol and water several times. The pre-synthesized nanowires were frozen in liquid nitrogen (–196 °C) and then freeze-dried in a bulk tray dryer (Labconco Corporation, Kansas City, MO, USA) at a sublimating temperature of –48 °C and a pressure of 0.04 mbar.
3
Fabrication of Porous Bacterial Cellulose Films
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BC hydrogels (Hainan Yeguo Foods Co., Ltd., China) were washed by deionized water (DIW) thoroughly and then cut into 3 × 4 cm2 rectangle slices with a sharp blade. The sliced pieces of BC hydrogels were frozen in liquid nitrogen and then transferred into a bulk tray dryer (Labconco Corporation, USA) for the subsequent freeze-drying process. The freeze-dried BC aerogel was pyrolyzed under the Ar atmosphere in a tubular furnace to get PBC film. Briefly, the temperature was slowly increased to 350 °C at a rate of 1 °C min−1 and held at this temperature for 1 h to stabilize the BC structure. And then, the temperature was continuously increased to 1000 °C at a rate of 3 °C min−1 and held at this temperature for 1 h to complete the carbonization process. After the carbonization process, the thickness of PBC film is 0.06 mm.
4
Lyophilized Urine Collection Pipettes
5
Antifungal Assay on Apple Agar
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For assays in solid media, apple agar was prepared using lyophilized apple powder (30 g/L) and bacteriological agar (20 g/L). Apple powder was achieved by cutting apples in pieces, froze them at -80 C for at least 3h and lyophilizing the pieces in a Bulk Tray Dryer with a 6-Port Manifold coupled to a FreeZone 6 Liter Console Freeze Dry System (Labconco, USA). The lyophilized pieces were then pulverized in a crusher. After dissolving these ingredients by stirring, the medium was sterilised at 121 ºC for 16 min.
To obtain the three batches with different PgAFP concentrations 0, 10 and 40 µg/mL, melted apple agar (45 ºC) was supplemented with a sterile solution of PgAFP prior to being poured into Petri plates. They were stored at room temperature until agar solidification. Then, plates were centrally inoculated with 2 µL from a spore suspension of P. expansum CMP-1 (10 6 conidia/mL) and incubated for 15 days at 25 ºC.
To obtain the three batches with different PgAFP concentrations 0, 10 and 40 µg/mL, melted apple agar (45 ºC) was supplemented with a sterile solution of PgAFP prior to being poured into Petri plates. They were stored at room temperature until agar solidification. Then, plates were centrally inoculated with 2 µL from a spore suspension of P. expansum CMP-1 (10 6 conidia/mL) and incubated for 15 days at 25 ºC.
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