Amphotericin B (AmB) (99.8% purity) and Nicardipine Hydrochloride (98% purity), the internal standard (IS), were purchased from Sigma–Aldrich (St. Louis, MO, USA different types of Poly(d ,l -lactide-co-glycolide) (Table 1 ) were supplied by Boehringer Ingelheim (Ingelheim, Germany). Miglyol®-812 neutral oil was obtained from Sasol (GmbH, Germany,). Vitamin E (TPGS-NF) grade was purchased from Peboc (Eastman, UK). Pluronic F68 Prilled was obtained from Ruger Chemical Co. Inc. (Irvington, New Jersey, USA). All other reagents and chemicals were HPLC analytical grade, and were used as received. Water was deionized and purified by a Milli-Q Reagent Grade water system from Millipore Corporation (Bedford, MX, USA).
Composition of the use pegylated PLGA–PEG copolymers types.
| Symbol | Name | Polymer type | Composition | Lactic to glycolic acid ratio | Content of PEG (%) | Molecular weight (Dalton) |
|---|---|---|---|---|---|---|
| A | RGPd 50105 | Diblock | PLGA–PEG 6000 | 1:1 | 10 | 5000 |
| B | RGPt 50106 | Triblock | PLGA–PEG 6000-PLGA | 1:1 | 10 | 6000 |
| C | RGPd 50155 | Diblock | PLGA–PEG 6000 | 1:1 | 15 | 6000 |
| D | RGPd 5055 | Diblock | PLGA–PEG 6000 | 1:1 | 5 | 5000 |
| E7 | R 203 H | Monoblock | Poly( | – | – | 18,000–28,000 |