GBM cells (LN18, U87MG, and U118MG) were procured from ATCC (Manassas, VA). LN18 cells were cultured in DMEM supplemented with 5% FBS, U87MG cells in Eagle’s MEM supplemented with 10% FBS, and U118MG cells in DMEM supplemented with 10% FBS. U87MG-LUC cells were constructed by transducing U87MG cells with MSCV Luciferase PGK, and clones were selected with hygromycin. AsA, rutin hydrate, MTT reagent, temozolomide (TMZ), crystal violet, haematoxylin and eosin were from Sigma-Aldrich (St. Louis, MO). Trypan blue was from Invitrogen (Carlsbad, CA). Antibodies for cleaved PARP, cleaved caspases (3, 9, and 8), Bid, Bad, GRP78, IRE1α, Calnexin, PDI, and Calpain were from Cell signaling (Danvers, MA). Survivin antibody was from Novus (Littleton, CO). α-Tubulin antibody was from Neomarkers (Fremont, CA). ECL detection system and anti-mouse HRP conjugated secondary antibody were from GE Healthcare (Buckinghamshire, UK). Protein assay kit was from Bio-Rad Laboratories (Hercules, CA). BAPTA and Fluo-3/AM were from Calbiochem (San Diego, CA). All other reagents were acquired in their highest purity grade available commercially.
Survivin antibody
Manufactured by Novus Biologicals
Survivin antibody is an affinity-purified polyclonal antibody that recognizes the Survivin protein. Survivin is a member of the inhibitor of apoptosis (IAP) gene family, which plays a role in the regulation of cell division and cell death.
Automatically generated - may contain errors
Lab products found in correlation
U87mg, by American Type Culture Collection (1 mentions)
Protein assay kit, by Bio-Rad (1 mentions)
Mtt reagent, by Merck Group (1 mentions)
Trypan blue, by Thermo Fisher Scientific (1 mentions)
U118mg, by American Type Culture Collection (1 mentions)
Rutin hydrate, by Merck Group (1 mentions)
Temozolomide tmz, by Merck Group (1 mentions)
Haematoxylin, by Merck Group (1 mentions)
Hrp conjugated anti mouse secondary antibody, by GE Healthcare (1 mentions)
Calnexin, by Cell Signaling Technology (1 mentions)
Ecl detection system, by GE Healthcare (1 mentions)
Ire1α, by Cell Signaling Technology (1 mentions)
P stat3 tyr705, by Cell Signaling Technology (1 mentions)
Ripa buffer, by Santa Cruz Biotechnology (1 mentions)
β actin antibody, by Merck Group (1 mentions)
P stat3 ser727, by Cell Signaling Technology (1 mentions)
2 protocols using survivin antibody
1
Glioblastoma Multiforme Cell Lines: Characterization and Treatment
2
Analyzing Apoptosis-related Proteins
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Total cell lysates were prepared using radioimmunoprecipitation (RIPA) buffer (Santa Cruz Biotechnology). Immunoblotting analysis caspase-3, cleaved caspase-3 followed a standard procedure. Antibodies against PARP, phosphorylated Stat3 (p-Stat3, Ser727), and p-Stat3 (Tyr705) were purchased from Cell Signaling (Danvers, MA); survivin antibody was purchased from Novus Biologicals (Littleton, CO); β-actin antibody was purchased from Sigma-Aldrich (St. Louis, MO).
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