Cells were fixed with 2.5% glutaraldehyde at 4 °C for 10 min and subsequently centrifuged at 2000 rpm for 10 min. The cell pellets were post-fixed with 3% glutaraldehyde. After post-fixation, the cell pellets were rinsed twice in H2O at 4 °C, dehydrated through a graded series of alcohol at 4–20 °C, infiltrated with graded mixtures of propylene oxide (substituted by acetone in 2 samples) and Epon at 20 °C, and embedded in 100% Epon at 30 °C. The ultra-thin sections were cut in three depths separated by 150 nm. The sections were contrasted with uranyl acetate and lead citrate and examined and photographed in a pre-calibrated Philips EM 208 electron microscope and a Megaview III FW camera (both FEI Company, Eindhoven, the Netherlands).
Megaview 3 fw camera
Manufactured by Thermo Fisher Scientific
The Megaview III FW camera is a high-performance digital camera designed for scientific and industrial imaging applications. It features a large, high-resolution image sensor and advanced image processing capabilities, delivering high-quality, detailed images.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using megaview 3 fw camera
1
Ultrastructural Analysis of Cells
2
Microparticle-myoblast Interaction Ultrastructure
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Microparticles cultured with proliferating myoblasts were spun down to a pellet and resuspended in 2% glutaraldehyde in 0.04 M phosphate buffer, pH 7.4 for 60 min at 20°C. The cells were spun down again and washed in 0.1 M phosphate buffer for 10 min. The pellet was resuspended in 15% bovine serum albumin (BSA) for 60 min at 20°C, and then incubated in 2% glutaraldehyde over night at 4°C to solidify.
Following rinsing, the solidified material/pellet was post-fixed with 1% osmium tetroxide in 0.1 M phosphate buffer for 60 min at 4°C, dehydrated through a graded series of alcohol and acetone at 4°C–20°C, and infiltrated in Epon for 90 min at 20°C. Ultra-thin sections were cut from the Epon block on a Leica Ultracut UCT ultramicrotome (Rowako AB), contrasted with uranyl acetate and lead citrate, and examined and photographed in a precalibrated Philips EM 208 electron microscope and a Megaview III FW camera (both FEI Company).
Following rinsing, the solidified material/pellet was post-fixed with 1% osmium tetroxide in 0.1 M phosphate buffer for 60 min at 4°C, dehydrated through a graded series of alcohol and acetone at 4°C–20°C, and infiltrated in Epon for 90 min at 20°C. Ultra-thin sections were cut from the Epon block on a Leica Ultracut UCT ultramicrotome (Rowako AB), contrasted with uranyl acetate and lead citrate, and examined and photographed in a precalibrated Philips EM 208 electron microscope and a Megaview III FW camera (both FEI Company).
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