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C18 silica

Manufactured by Agilent Technologies
Sourced in United States

C18 silica is a type of stationary phase used in high-performance liquid chromatography (HPLC) and other chromatographic techniques. It consists of silica particles that have been chemically bonded with octadecyl (C18) hydrocarbon chains. The C18 functional groups provide a hydrophobic surface that interacts with non-polar or hydrophobic analytes, allowing for their separation and analysis.

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2 protocols using c18 silica

1

Synthesis and Purification of 13C-Labeled Coniferyl Alcohol

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[β-13C]-Ferulic acid was prepared from vanillin and [2-13C]-malonic acid via Knoevenagel condensation with anhydrous pyridine and aniline, following a published method,23,24 (link) in 86% yield. 1H NMR data were identical with unlabelled ferulic acid; δC (100 MHz, CD3OD) 115.8 ppm; ESI m/z 193.0 (MH). [β-13C]-Ferulic acid was converted to [β-13C]-coniferyl alcohol using the method of Tramontina et al.,25 (link) using E. coli cells overexpressing Nocardia iowensis carboxylic acid reductase (NiCAR) and Coptotermes gestroi aldo–keto reductase (CgAKR-1). The [β-13C]-coniferyl alcohol product was purified using solid phase extraction. A column (2 × 30 cm) containing 10 g of C18 silica (Agilent) was conditioned using methanol (30 mL) followed by water (30 mL). Samples were applied into the column at a flow rate of 15 mL min−1; which was washed with water (30 mL); then coniferyl alcohol was eluted using methanol (30 mL). Yield 70%. 1H NMR spectral data were identical to unlabelled coniferyl alcohol,23,24 (link) and matched literature data for [β-13C]-coniferyl alcohol.15,24 (link)δC (100 MHz, CD3OD) 125.6 ppm; ESI m/z 179.0 (MH).
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2

Quantitative Analysis of Polycyclic Musks

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Standard solutions of five polycyclic musks, namely, celestolide (ADBI), phantolide (AHMI), traseolide (ATII), galaxolide (HHCB), and tonalide (AHTN), with concentrations of 10 mg/L were obtained from Dr. Ehrenstorfer (Augsburg, Germany). The internal standard, 13C6-labeled hexachlorobenzene (13C6-HCB, 100 mg/L), was obtained from Cambridge Isotope Laboratories (Andover, MA, USA). Pesticide-grade n-hexane, dichloromethane (DCM), acetone, and chromatography-grade ethyl acetate were supplied by Fisher Scientific (J.T. Baker, Pittsburgh, PA, USA). N,N′-Dicyclohexylcarbodiimide (DCC) was obtained from Alfa Aesar (Ward Hill, MA, USA). Hydrazine hydrate (85%) was purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Analytical-grade N,N-dimethylformamide (DMF) was obtained from J&K Scientific Ltd. (Beijing, China).
Graphene oxides (GOs) were purchased from Nanjing XFNANO Materials Tech (Nanjing, China). Aminosilica and C18 silica were obtained from Agilent (Santa Clara, CA, USA). N-Vinylpyrrolidone polymeric cartridges (Oasis HLB) were obtained from Waters (Milford, MA, USA).
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