Anti human cd3 bv510

Manufactured by BioLegend

Sourced in United States

Anti-human CD3-BV510 is a fluorochrome-conjugated monoclonal antibody that binds to the CD3 antigen, which is expressed on the surface of human T cells. The BV510 fluorochrome is used for flow cytometric analysis.

Automatically generated - may contain errors

Lab products found in correlation

Cd25 pe cy7, by BD (2 mentions) Anti human foxp3 ef450, by Thermo Fisher Scientific (2 mentions) Cd127 af647, by BioLegend (2 mentions) Cd4 fitc, by Thermo Fisher Scientific (2 mentions) Facsaria 3, by BD (2 mentions)

2 protocols using anti human cd3 bv510

Isolation of Treg and non-Treg Subsets

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For TCR sequencing purposes, CD3⁺CD4⁺CD25^highCD127^low Tregs and CD3⁺CD4+CD25^low/intCD127^int/high non-Tregs were isolated from frozen PBMCs and SFMCs, using the FACS Aria III (BD, Franklin Lakes, NJ). Antibodies used for sorting were: anti-human CD3-BV510 (BioLegend, San Diego, CA), CD4-FITC (eBioscience, Frankfurt am Main, Germany), CD25-PE/Cy7 (BD), and CD127-AF647 (BioLegend). To check for FOXP3 expression of the sorted populations anti-human FOXP3-eF450 (eBioscience) was used.

Mijnheer G., Servaas N.H., Leong J.Y., Boltjes A., Spierings E., Chen P., Lai L., Petrelli A., Vastert S., de Boer R.J., Albani S., Pandit A, & van Wijk F. (2023). Compartmentalization and persistence of dominant (regulatory) T cell clones indicates antigen skewing in juvenile idiopathic arthritis. eLife, 12, e79016.

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Sorting Tregs and Non-Tregs from PBMC/SFMC

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For TCR sequencing purposes, CD3 + CD4 + CD25 high CD127 low Tregs and CD3 + CD4+CD25 low/int CD127 int/high non-Tregs were isolated from frozen PBMC and SFMC, using the FACS Aria III (BD, Franklin Lakes, New Jersey, United States).
Antibodies used for sorting were: anti human CD3-BV510 (Biolegend, San Diego, California, United States), CD4-FITC (eBioscience, Frankfurt am Main, Germany), CD25-PE/Cy7 (BD), CD127-AF647 (Biolegend). To check for FOXP3 expression of the sorted populations anti human FOXP3-eF450 (eBioscience) was used.

Mijnheer G., Servaas N.H., Leong J.Y., Boltjes A., Spierings E., Chen P., Lai L., Petrelli A., Vastert S.J., Boer R.J.d., Albani S., Pandit A., & Wijk F.v. (2022). Compartmentalization and persistence of dominant (regulatory) T cell clones indicates antigen skewing in juvenile idiopathic arthritis.

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