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Ar solution

Manufactured by Lifeline Cell Technology
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2% AR solution is a laboratory product that provides a 2% concentration of Arachidonic Acid (AR) in a suitable solvent. The core function of this solution is to serve as a reagent or substrate for various cell culture, biochemical, and analytical applications that require a stable and consistent source of Arachidonic Acid.

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Lab products found in correlation

Stempro osteogenic differentiation kit, by Thermo Fisher Scientific (2 mentions)

2 protocols using ar solution

1

In Vitro Osteogenic Differentiation of NCC-MPCs

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At passage 4–5, NCC-MPCs were detached and dissociated into single cells using pre-warmed TrypLE™ Express and plated into 12-well culture with a plating density of 104 cells/cm2. Cells were incubated with serum-containing media for 2–4 h, which was then replaced with osteogenic differentiation media (StemPro Osteogenic differentiation kit, Life Technologies) and re-fed every 3–4 days. After 18–23 days, cells were stained using Alizarin Red stain. For Alizarin Red staining, cells were fixed with 4% paraformaldehyde for 30 min, rinsed twice with distilled water, and stained with 2% AR solution (Lifeline Cell Technology) for 2–3 min. Cells were rinsed 3 times with distilled water and visualized under light microscope for image capturing and analysis.
Jamal M., Lewandowski S.L., Lawton M.L., Huang G.T, & Ikonomou L. (2018). Derivation and characterization of putative craniofacial mesenchymal progenitor cells from human induced pluripotent stem cells. Stem cell research, 33, 100-109.
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2

In Vitro Osteogenic Differentiation of NCC-MPCs

Check if the same lab product or an alternative is used in the 5 most similar protocols
At passage 4–5, NCC-MPCs were detached and dissociated into single cells using pre-warmed TrypLE™ Express and plated into 12-well culture with a plating density of 104 cells/cm2. Cells were incubated with serum-containing media for 2–4 h, which was then replaced with osteogenic differentiation media (StemPro Osteogenic differentiation kit, Life Technologies) and re-fed every 3–4 days. After 18–23 days, cells were stained using Alizarin Red stain. For Alizarin Red staining, cells were fixed with 4% paraformaldehyde for 30 min, rinsed twice with distilled water, and stained with 2% AR solution (Lifeline Cell Technology) for 2–3 min. Cells were rinsed 3 times with distilled water and visualized under light microscope for image capturing and analysis.
Jamal M., Lewandowski S.L., Lawton M.L., Huang G.T, & Ikonomou L. (2018). Derivation and characterization of putative craniofacial mesenchymal progenitor cells from human induced pluripotent stem cells. Stem cell research, 33, 100-109.
+ Open protocol
+ Expand

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