Apc cd11c

Manufactured by Bio-Rad

The APC-CD11c is a fluorescent-labeled antibody used in flow cytometry to detect the CD11c cell surface marker. CD11c is expressed on the surface of dendritic cells, macrophages, and other myeloid cells. The APC-CD11c antibody can be used to identify and characterize these cell populations.

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2 protocols using apc cd11c

Multiparametric Immune Cell Profiling

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Surface and intracellular staining were performed as described previously [27 (link)] using the indicated antibodies. From BioLegend: Alexa fluor 488-Ly6C, PE-Cy7-Ly6G, PE-Cy7-streptavidin, APC-streptatividin; From BD Pharmigen: APC-CD11b, PE-Cy7 anti-mouse CD11c, Rat anti-mouse CD16/CD32, PE-conjugated anti-Gr-1, Alexa 647-conjugated anti- PY418 SRC, PE-conjugated ant-AKT1, from Invitrogen: APC-CD11c, PE-streptavidin, FITC-streptavidin; from AbDSerotec: PE-CD62L; From Santa Cruz Biotechnology: PE-conjugated anti-Fyn. From Cell Signaling, Alexa 647-conjugated anti-SRC rabbit antibody (clone 36D10) and PE-conjugated anti-phospho AKT rabbit antibody (Ser473, clone D9E).

Gato-Cañas M., de Morentin X.M., Blanco-Luquin I., Fernandez-Irigoyen J., Zudaire I., Liechtenstein T., Arasanz H., Lozano T., Casares N., Chaikuad A., Knapp S., Guerrero-Setas D., Escors D., Kochan G, & Santamaría E. (2015). A core of kinase-regulated interactomes defines the neoplastic MDSC lineage. Oncotarget, 6(29), 27160-27175.

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Multiparametric Flow Cytometry Analysis

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Surface and intracellular staining were performed as described previously using the indicated antibodies [33 (link)]. From BioLegend: Alexa fluor 488-Ly6C, PE-CD3, PE-CD86, APC-CD80, APC-I-A/I-F, Biotin-ICAM I, Biotin-H2kb, Alexa fluor 488-IFN-γ, Biotin-PD-L1, PE-Cy7-Ly6G, PE-CD34, PE-CD14, PE-F4/80, PE-Cy7-streptavidin, APC-streptatividin; From eBioscience: PE-CD4, FITC-IA/IE; From Raybiotech: FITC-class I H-2Kb; From BD bioscience pharmigen: v500-CD4, PE-CD8alpha, APC-CD11b, AF647-Ki67; from Southernbiotech: from Invitrogen: APC-CD11c; PE-streptavidin, FITC-streptavidin; from AbD Serotec: PE-CD62L; from R&D Systems: anti-human/mouse PE-Arginase 1. Viability was established by flow cytometry using the Fixable viability dye-eF780 from eBioscience. From Santa Cruz Biotechnology, NOS2-PE. When indicated, cells samples were treated overnight with 100 ng/ml lipololysaccharide (LPS) from Salmonella enterica serotype abortusequi (SIGMA).

Liechtenstein T., Perez-Janices N., Gato M., Caliendo F., Kochan G., Blanco-Luquin I., Van der Jeught K., Arce F., Guerrero-Setas D., Fernandez-Irigoyen J., Santamaria E., Breckpot K, & Escors D. (2014). A highly efficient tumor-infiltrating MDSC differentiation system for discovery of anti-neoplastic targets, which circumvents the need for tumor establishment in mice. Oncotarget, 5(17), 7843-7857.

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