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P 500

Manufactured by Cytiva

The P-500 is a liquid chromatography pump designed for laboratory use. It provides precise and reliable flow control for a variety of chromatographic applications. The P-500 can deliver flow rates from 0.001 to 50 mL/min, with a maximum pressure of 50 MPa.

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2 protocols using p 500

1

Plasma Metabolite Profiling in Mice

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Blood was collected by puncture of the v.facialis from ad libitum fed or 12 h-fasted mice. TG, TC (DiaSys, Holzheim, Germany), and β-hydroxybutyrate (Cayman, Ann Harbor, MI) concentrations were measured enzymatically according to manufacturer's instructions. Lipoprotein fractions from 200 μl pooled plasma per genotype were separated using fast protein liquid chromatography (Pharmacia P-500) equipped with a Superose 6 column (Amersham Biosciences, Piscataway, NJ) and TG and TC concentrations were assayed spectrophotometrically. Alanine aminotransferase and aspartate aminotransferase levels were measured enzymatically (Roche Diagnostics, Risch, Switzerland). Blood glucose concentration was determined using Accu-Chek® Active glucometer and glucose strips (Roche Diagnostics).
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2

Plasma Metabolite Profiling in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols
Blood was collected by puncture of the v.facialis from ad libitum fed or 12 h-fasted mice. TG, TC (DiaSys, Holzheim, Germany), and β-hydroxybutyrate (Cayman, Ann Harbor, MI) concentrations were measured enzymatically according to manufacturer's instructions. Lipoprotein fractions from 200 μl pooled plasma per genotype were separated using fast protein liquid chromatography (Pharmacia P-500) equipped with a Superose 6 column (Amersham Biosciences, Piscataway, NJ) and TG and TC concentrations were assayed spectrophotometrically. Alanine aminotransferase and aspartate aminotransferase levels were measured enzymatically (Roche Diagnostics, Risch, Switzerland). Blood glucose concentration was determined using Accu-Chek® Active glucometer and glucose strips (Roche Diagnostics).
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