Human ki67

Manufactured by Agilent Technologies

The Human Ki67 is a laboratory equipment product that measures the expression of the Ki67 protein, which is a cellular marker for proliferation. It provides information about the cell cycle and cell division activity in biological samples.

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Lab products found in correlation

Cleaved caspase 3, by Cell Signaling Technology (2 mentions) Dp2 bsw software, by Olympus (1 mentions) Vimentin, by Leica (1 mentions) β catenin, by BD (1 mentions) Liquid dab, by Agilent Technologies (1 mentions) Dako antibody diluent, by Agilent Technologies (1 mentions) Lamc1, by Merck Group (1 mentions) Phospho histone h3 ser10 antibody, by Cell Signaling Technology (1 mentions)

Spelling variants of this product

Mouse anti-human Ki67 (7 citations) Anti-human Ki67 monoclonal MIB1 antibody (3 citations) Anti-human Ki67 monoclonal antibody (2 citations)

3 protocols using human ki67

Immunohistochemical Analysis of Brain Tumors

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Mice bearing tumors were perfused with formalin and brains were removed and sectioned. Brain sections were immunostained with antibodies against human Ki67 (DAKO, Carpinteria, CA), cleaved caspase-3 (Cell Signaling Technology) as described earlier [8 (link)]. Photomicrographs of immunohistochemistry and hematoxylin and eosin slides were processed using DP2-BSW Software (Olympus).

Redjal N., Zhu Y, & Shah K. (2015). Combination of Systemic Chemotherapy with Local Stem Cell Delivered S-TRAIL in Resected Brain Tumors. Stem cells (Dayton, Ohio), 33(1), 101-110.

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Immunohistochemical Analysis of Xenografts

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Xenografts were incubated in 4% paraformaldehyde for 24 h at 4 °C then remained in 70% ethanol until processed for paraffin wax embedding by the in-house histology core facility. Antibodies used were as follows: β-catenin (BD Biosciences 610,154, 1:100); cleaved caspase-3 (Cell Signaling Technology 9664, 1:200); E-cadherin (Daki M3612, 1:25); human Ki67 (Dako M7240, 1:400); Vimentin (Novocastra NCL-L-VIM-572, 1:400); and secondary antibodies from the Bond Polymer Refine Kit.

Junaid F., Tomic G., Kemp R, & Winton D.J. (2023). Single-copy Snail upregulation causes partial epithelial-mesenchymal transition in colon cancer cells. BMC Cancer, 23, 153.

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Immunohistochemical Staining of Paraffin Sections

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Paraffin-embedded tissue sections (4 μm) were deparaffinized in xylene and rehydrated in graded alcohols. Antigen retrieval was performed by incubating tissue sections with TrilogyTM (Cell Marque, Austin, TX, catalog # CMX833). Endogenous peroxidase activity was blocked by incubation with 3% H₂O₂ for 15 min. Sections were pre-incubated with Dako Antibody Diluent (Dako, Carpinteria, CA) at room temperature for 30 min, followed by incubation with antibody diluted in Dako Antibody Diluent at 4°C overnight. Positive reactions were detected by applying EnVision™+/HRP polymer (Dako) for 30 min, followed by incubation in DAB substrate for 5 min (Liquid DAB+, Dako). The slides were then counterstained with hematoxylin to visualize the cell nuclei. Antibodies used were: human Ki-67 (cat # M7240, Dako), mouse Ki-67 (cat # 12202, Cell Signaling Technology), LAMC1 (cat # HPA001908, Sigma-Aldrich), and phospho-Histone H3 (Ser10) antibody (cat # 9701, Cell Signaling Technology).

Kobayashi Y., Kashima H., Wu R.C., Jung J.G., Kuan J.C., Gu J., Xuan J., Sokoll L., Visvanathan K., Shih I.M, & Wang T.L. (2015). Mevalonate Pathway Antagonist Inhibits Proliferation of Serous Tubal Intraepithelial Carcinoma and Ovarian Carcinoma in Mouse Models. Clinical cancer research : an official journal of the American Association for Cancer Research, 21(20), 4652-4662.

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