Standard IHC techniques were used to probe intestinal tissues for zonulin expression using the following antibodies: sheep anti-mouse haptoglobin primary antibody (Invitrogen PA5-33158); rabbit anti-sheep HRP conjugated secondary antibody (Abcam Ab97130). Details of the experimental methods can be found in online supplementary material . All images used in this study have been uploaded to the Mouse Model of Type 1 Diabetes Atlas (MMDA)30 and are available for viewing and discussion online (mmda.lib.miamioh.edu).
Pa5 33158
Manufactured by Thermo Fisher Scientific
The PA5-33158 is a laboratory equipment product manufactured by Thermo Fisher Scientific. It is designed to perform specific functions within a laboratory setting. As a marketing specialist, I can provide a concise, factual, and unbiased description of the core function of this product, without extrapolation or interpretation.
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Lab products found in correlation
2 protocols using pa5 33158
1
Intestinal Zonulin Expression Profiling
2
Antibody Validation via Western Blot
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Standard Western blots were used to confirm reactivity of the antibodies to haptoglobin and zonulin using the following antibodies: sheep anti-mouse haptoglobin primary (Invitrogen PA5-33158); rabbit anti-sheep horseradish peroxidase (HRP) conjugated secondary (Abcam Ab97130). Detailed methods for the Western blot experiments can be found in online supplementary material .
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